Please use this identifier to cite or link to this item: https://hdl.handle.net/1822/51667

TitleArabidopsis Squalene Epoxidase 3 (SQE3) Complements SQE1 and is important for embryo development and bulk squalene epoxidase activity
Author(s)Laranjeira, Sara Catarina Costa
Amorim-Silva, Vitor
Esteban, Alicia
Arró, Monserrat
Ferrer, Albert
Tavares, R. M.
Botella, Miguel Angel
Rosado, Abel
Azevedo, Herlânder Anselmo Queirós Pereira
KeywordsArabidopsis
Endoplasmic Reticulum
Gene Expression Regulation, Plant
Genetic Complementation Test
Mutation
Phylogeny
Protein Transport
Seeds
Squalene Monooxygenase
embryo development
MVA pathway
squalene epoxidase
sterol biosynthesis
Issue dateJul-2015
PublisherCell Press
JournalMolecular Plant
Abstract(s)The existence of multigenic families in the mevalonate pathway suggests divergent functional roles for pathway components involved in the biosynthesis of plant sterols. Squalene epoxidases (SQEs) are key components of this pathway, and Squalene Epoxidase 1 (SQE1) has been identified as a fundamental enzyme in this biosynthetic step. In the present work, we extended the characterization of the remaining SQE family members, phylogenetically resolving between true SQEs and a subfamily of SQE-like proteins that is exclusive to Brassicaceae. Functional characterization of true SQE family members, Squalene Epoxidase 2 (SQE2) and Squalene Epoxidase 3 (SQE3), indicates that SQE3, but not SQE2, contributes to the bulk SQE activity in Arabidopsis, with sqe3-1 mutants accumulating squalene and displaying sensitivity to terbinafine. We genetically demonstrated that SQE3 seems to play a particularly significant role in embryo development. Also, SQE1 and SQE3 both localize in the endoplasmic reticulum, and SQE3 can functionally complement SQE1. Thus, SQE1 and SQE3 seem to be two functionally unequal redundant genes in the promotion of plant SQE activity in Arabidopsis.
TypeArticle
URIhttps://hdl.handle.net/1822/51667
DOI10.1016/j.molp.2015.02.007
ISSN1674-2052
Peer-Reviewedyes
AccessRestricted access (Author)
Appears in Collections:CBFP - Artigos/Papers
DBio - Artigos/Papers

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