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https://hdl.handle.net/1822/52720
Título: | Immobilization of bacteriophage in wound-dressing nanostructure |
Autor(es): | Nogueira, F. Karumidze, Natia Kusradze, Ia Goderdzishvili, Marina Teixeira, Pilar Gouveia, Isabel C. |
Data: | Nov-2017 |
Editora: | Elsevier |
Revista: | Nanomedicine: Nanotechnology, Biology, and Medicine |
Citação: | Nogueira, F.; Karumidze, Natia; Kusradze, Ia; Goderdzishvili, Marina; Teixeira, Pilar; Gouveia, Isabel C., Immobilization of bacteriophage in wound-dressing nanostructure. Nanomedicine-Nanotechnology Biology and Medicine, 13(8), 2475-2484, 2017 |
Resumo(s): | Opportunistic bacteria that cause life-threatening infections are still a central problem associated with a healthcare setting. Bacteriophage capsid immobilization on nanostructured polymers maximizes its tail exposure and looks promising in applications toward skin-infections as alternative to antibiotics standardly used. The main goal of this work was to investigate the covalent immobilization of vB_Pae_Kakheti25 bacteriophage capsid on polycaprolactone (PCL) nanofibers (non-woven textile), as a potential effective antimicrobial, laundry resistant and non-toxic dressing for biomedical use. Surface analyses showed that the immobilization of vB_Pae_Kakheti25 bacteriophage capsid on PCL nanofibres oriented bacteriophage tails to interact with bacteria. Furthermore, antimicrobial assays showed a very effective 6 log bacterial reduction, which was equivalent to 99.9999%, after immediate and 2 hours of contact, even following 25 washing cycles (due to covalent bond). The activity of PCL-vB_Pae_Kakheti25 against P. aeruginosa was immediate and its reduction was complete. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/52720 |
DOI: | 10.1016/j.nano.2017.08.008 |
ISSN: | 15499634 |
e-ISSN: | 1549-9642 |
Versão da editora: | http://www.nanomedjournal.com/ |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_46948_1.pdf Acesso restrito! | 2,07 MB | Adobe PDF | Ver/Abrir |