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https://hdl.handle.net/1822/5332
Título: | Comparative antibacterial potential of selected aldehyde-based biocides and surfactants against planktonic Pseudomonas fluorescens |
Autor(es): | Simões, M. Pereira, Maria Olívia Machado, Idalina Simões, Lúcia C. Vieira, M. J. |
Palavras-chave: | Antimicrobial effect Biocide Cetyltrimethyl ammonium bromide European Standard EN-1276 Glutaraldehyde Ortho-phthalaldehyde Resistance Sodium dodecyl sulphate Surfactant |
Data: | Set-2006 |
Editora: | Springer |
Revista: | Journal of Industrial Microbiology and Biotechnology |
Citação: | "Journal of Industrial Microbiology and Biotechnology". ISSN 1367-5435. 33:9 (2006) 741-749. |
Resumo(s): | The antimicrobial efficacy of two aldehydebased biocides (glutaraldehyde, GTA, and orthophthalaldehyde, OPA) and two surfactants (cetyltrimethyl ammonium bromide, CTAB, and sodium dodecyl sulphate, SDS) was tested against planktonic Pseudomonas fluorescens. The antimicrobial effects were evaluated by respiratory activity as a measure of the oxygen uptake rate, adenosine triphosphate (ATP) release, outer membrane proteins (OMP) expression and cellular colour changes. The results were compared with the bacterial characteristics without chemical treatment. Tests in the presence of bovine serum albumin (BSA), in order to mimic a disinfection process in the real situation under dirty conditions, were performed according to the European Standard EN-1276. P. fluorescens was completely inactivated with OPA (minimum bactericidal concentration, MBC = 0.5 mM) and CTAB (MBC = 5 mM) and was resistant to GTA and SDS. Only CTAB promoted cellular disruption and consequent ATP release. The antimicrobial action of the chemicals tested was significantly reduced when BSA was introduced into the bacterial cultures, increasing markedly the MBC values. Additionally, the presence of BSA acted as a disruption protective agent when CTAB was applied and stimulated the bacterial respiratory activity when lower concentrations of SDS were tested. The OMP of the bacterial cells was affected by the application of both surfactants. OMP expression remained unaltered after biocide treatment. Bacterial colour change was noticed after treatment with biocides and surfactants. In summary, P. fluorescens was extremely resistant to GTA and SDS, with antimicrobial action being quenched markedly by the reaction with BSA. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/5332 |
DOI: | 10.1007/s10295-006-0120-5 |
ISSN: | 1367-5435 |
Versão da editora: | http://www.springer.com/east/home?SGWID=5-102-70-1173835-0&SHORTCUT=www.springer.com/10295 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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JIMB2006-Simoes[1].pdf | 317,4 kB | Adobe PDF | Ver/Abrir |