Optimization of bromelain isolation from pineapple byproducts by polysaccharide complex formation

doi:10.1016/j.foodhyd.2018.09.009

Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/56412

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Campo DC	Valor	Idioma
dc.contributor.author	Campos, Débora A.	por
dc.contributor.author	Coscueta, Ezequiel R.	por
dc.contributor.author	Valetti, Nadia Woitovich	por
dc.contributor.author	Pastrana-Castro, Lorenzo M.	por
dc.contributor.author	Teixeira, J. A.	por
dc.contributor.author	Picó, Guillermo A.	por
dc.contributor.author	Pintado, Maria Manuela	por
dc.date.accessioned	2018-10-19T17:19:33Z	-
dc.date.available	2018-10-19T17:19:33Z	-
dc.date.issued	2019	-
dc.identifier.citation	Campos, Débora A.; Coscueta, Ezequiel R.; Valetti, Nadia Woitovich; Pastrana-Castro, Lorenzo M.; Teixeira, José A.; Picó, Guillermo A.; Pintado, Maria Manuela, Optimization of bromelain isolation from pineapple byproducts by polysaccharide complex formation. Food Hydrocolloids, 87, 792-804, 2019	por
dc.identifier.issn	0268-005X	por
dc.identifier.uri	https://hdl.handle.net/1822/56412	-
dc.description	Supplementary data related to this article can be found at https:// doi.org/10.1016/j.foodhyd.2018.09.009.	por
dc.description.abstract	A simple method for bromelain extraction from industrial pineapple residues (stems and peels) was developed and optimized through factorial experimental design. The developed methodology, based on precipitation with carrageenan, represents an alternative to the use of organic solvents and inorganic salts (common industrial precipitation) and allows achieving extracts with high bromelain purity. High recovery yield 8090% - of active bromelain was obtained for both crude juices (stems and peels) making possible to obtain ca. 0.3g of bromelain from 100g of pineapple byproducts using a low polysaccharide concentration (0.20.3% w/v).	por
dc.description.sponsorship	This work was ﬁnancially supported by BiValBi - Biotechnologies to Valorise the regional Biodiversity in Latin America, in the funding project FP7-PEOPLE-2013-IRSES PEOPLE Marie Curie Programthrough project reference PIRSES-GA-2013-611493. We would like to thank the scientiﬁc collaboration of CBQF under the FCT – Fundação para a Ciência e a Tecnologia through project Multibioreﬁnery – Multi-purpose strategies for the valorization of a wide range of agroforestry byproducts and ﬁsheries: A step forward in the creation of an integrated bioreﬁnery, (POCI-01-0145-FEDER-0066), project UID/Multi/50016/ 2013 and by PhD grant SFRH/BD/104074/2014 to Débora Campos.	por
dc.language.iso	eng	por
dc.publisher	Elsevier	por
dc.relation	info:eu-repo/grantAgreement/FCT/5876/147263/PT	por
dc.relation	SFRH/BD/104074/2014	por
dc.relation	PIRSES-GA-2013-611493	por
dc.relation	info:eu-repo/semantics/dataset/doi/10.1016/j.foodhyd.2018.09.009	por
dc.rights	openAccess	por
dc.subject	Protein complex	por
dc.subject	Natural crude juices	por
dc.subject	Carrageenan	por
dc.subject	Bromelain	por
dc.title	Optimization of bromelain isolation from pineapple byproducts by polysaccharide complex formation	por
dc.type	article	-
dc.peerreviewed	yes	por
dc.relation.publisherversion	http://www.elsevier.com/locate/issn/0268005X	por
dc.comments	CEB48953	por
oaire.citationStartPage	792	por
oaire.citationEndPage	804	por
oaire.citationConferencePlace	Netherlands	-
oaire.citationVolume	87	por
dc.date.updated	2018-10-19T15:49:48Z	-
dc.identifier.doi	10.1016/j.foodhyd.2018.09.009	por
dc.description.publicationversion	info:eu-repo/semantics/publishedVersion	por
dc.subject.wos	Science & Technology	por
sdum.journal	Food Hydrocolloids	por
Aparece nas coleções:	CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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