Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/56620
Título: | Downscale fermentation for xylooligosaccharides production by recombinant Bacillus subtilis 3610 |
Autor(es): | Amorim, Cláudia Silvério, Sara C. Gonçalves, Raquel F. S. Pinheiro, Ana Cristina Silva, Soraia Coelho, Elisabete Coimbra, Manuel A. Prather, Kristala L. J. Rodrigues, L. R. |
Palavras-chave: | Xylooligosaccharides Bacillus subtilis prebiotic xylan xylanase |
Data: | Fev-2019 |
Editora: | Elsevier |
Revista: | Carbohydrate Polymers |
Citação: | Amorim, Cláudia; Silvério, Sara C.; Gonçalves, Raquel F. S.; Pinheiro, Ana Cristina; Silva, Soraia; Coelho, Elisabete; Coimbra, Manuel A.; Prather, Kristala L. J.; Rodrigues, Lígia R., Downscale fermentation for xylooligosaccharides production by recombinant Bacillus subtilis 3610. Carbohydrate Polymers, 205, 176-183, 2019 |
Resumo(s): | The global demand of prebiotics such as xylooligosaccharides (XOS) has been growing over the years, motivating the search for different production processes with increased efficiency. In this study, a cloned Bacillus subtilis 3610, containing the xylanase gene xyn2 of Trichoderma reesei coupled with an endogenous secretion tag, was selected for XOS production through direct fermentation of beechwood xylan. A mixture of XOS with a degree of polymerization ranging from 4-6 was obtained, presenting high stability after a static in vitro digestion (98.5±0.2%). The maximum production yield expressed as total XOS per amount of xylan (306±4mg/g) was achieved after 8h of fermentation operating under one-time impulse fed-batch. The optimal conditions found were pH 6.0 and 42.5°C, using 2.5g/L of initial concentration of xylan increased up to 5.0g/L at 3h. Xylopentaose was the major oligosaccharide produced, representing 47% of the total production yield. |
Tipo: | Artigo |
Descrição: | Supplementary material related to this article can be found, in the online version, at doi:https://doi.org/10.1016/j.carbpol.2018.09.088. |
URI: | https://hdl.handle.net/1822/56620 |
DOI: | 10.1016/j.carbpol.2018.09.088 |
ISSN: | 0144-8617 |
e-ISSN: | 0144-8617 |
Versão da editora: | http://www.journals.elsevier.com/carbohydrate-polymers/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_48969_1.pdf | 457,08 kB | Adobe PDF | Ver/Abrir |