Please use this identifier to cite or link to this item: https://hdl.handle.net/1822/58050

TitleL-Threonine Supplementation During Colitis Onset Delays Disease Recovery
Author(s)Gaifem, Joana
Gonçalves, Luís G.
Dinis-Oliveira, Ricardo J.
Cunha, Cristina
Carvalho, Agostinho
Torrado, Egídio
Rodrigues, Fernando José dos Santos
Saraiva, Margarida
Castro, António G.
Silvestre, Ricardo Jorge Leal
KeywordsIBD
Threonine
DSS-induced colitis
Goblet cells
Metabolomics
IL-22
Mucin
Issue date2018
PublisherFrontiers Media
JournalFrontiers in Physiology
CitationGaifem, J., Gonçalves, L. G., Dinis-Oliveira, R. J., Cunha, C., et. al. (2018). L-Threonine supplementation during colitis onset delays disease recovery. Frontiers in physiology, 9
Abstract(s)Dietary nutrients have emerged as potential therapeutic adjuncts for inflammatory bowel disease (IBD) given their impact on intestinal homeostasis through the modulation of immune response, gut microbiota composition and epithelial barrier stability. Several nutrients have already been associated with a protective phenotype. Yet, there is a lack of knowledge toward the most promising ones as well as the most adequate phase of action. To unveil the most prominent therapy candidates we characterized the colon metabolic profile during colitis development. We have observed a twofold decrease in threonine levels in mice subjected to DSS-induced colitis. We then assessed the effect of threonine supplementation in the beginning of the inflammatory process (DSS + Thr) or when inflammation is already established (DSS + Thr D8). Colitis progression was similar between the treated groups and control colitic mice, yet threonine had a surprisingly detrimental effect when administered in the beginning of the disease, with mice displaying a delayed recovery when compared to control mice and mice supplemented with threonine after day 8. Although no major changes were found in their metabolic profile, DSS + Thr mice displayed altered expression in mucin-encoding genes, as well as in goblet cell counts, unveiling an impaired ability to produce mucus. Moreover, IL-22 secretion was decreased in DSS + Thr mice when compared to DSS + Thr D8 mice. Overall, these results suggest that supplementation with threonine during colitis induction impact goblet cell number and delays the recovery period. This reinforces the importance of a deeper understanding regarding threonine supplementation in IBD.
TypeArticle
URIhttps://hdl.handle.net/1822/58050
DOI10.3389/fphys.2018.01247
ISSN1664-042X
Publisher versionhttps://www.frontiersin.org/articles/10.3389/fphys.2018.01247/full
Peer-Reviewedyes
AccessOpen access
Appears in Collections:ICVS - Artigos em revistas internacionais / Papers in international journals

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