Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/58050

Registo completo
Campo DCValorIdioma
dc.contributor.authorGaifem, Joanapor
dc.contributor.authorGonçalves, Luís G.por
dc.contributor.authorDinis-Oliveira, Ricardo J.por
dc.contributor.authorCunha, Cristinapor
dc.contributor.authorCarvalho, Agostinhopor
dc.contributor.authorTorrado, Egídiopor
dc.contributor.authorRodrigues, Fernando José dos Santospor
dc.contributor.authorSaraiva, Margaridapor
dc.contributor.authorCastro, António G.por
dc.contributor.authorSilvestre, Ricardo Jorge Lealpor
dc.date.accessioned2019-01-11T11:01:22Z-
dc.date.available2019-01-11T11:01:22Z-
dc.date.issued2018-
dc.identifier.citationGaifem, J., Gonçalves, L. G., Dinis-Oliveira, R. J., Cunha, C., et. al. (2018). L-Threonine supplementation during colitis onset delays disease recovery. Frontiers in physiology, 9por
dc.identifier.issn1664-042X-
dc.identifier.urihttps://hdl.handle.net/1822/58050-
dc.description.abstractDietary nutrients have emerged as potential therapeutic adjuncts for inflammatory bowel disease (IBD) given their impact on intestinal homeostasis through the modulation of immune response, gut microbiota composition and epithelial barrier stability. Several nutrients have already been associated with a protective phenotype. Yet, there is a lack of knowledge toward the most promising ones as well as the most adequate phase of action. To unveil the most prominent therapy candidates we characterized the colon metabolic profile during colitis development. We have observed a twofold decrease in threonine levels in mice subjected to DSS-induced colitis. We then assessed the effect of threonine supplementation in the beginning of the inflammatory process (DSS + Thr) or when inflammation is already established (DSS + Thr D8). Colitis progression was similar between the treated groups and control colitic mice, yet threonine had a surprisingly detrimental effect when administered in the beginning of the disease, with mice displaying a delayed recovery when compared to control mice and mice supplemented with threonine after day 8. Although no major changes were found in their metabolic profile, DSS + Thr mice displayed altered expression in mucin-encoding genes, as well as in goblet cell counts, unveiling an impaired ability to produce mucus. Moreover, IL-22 secretion was decreased in DSS + Thr mice when compared to DSS + Thr D8 mice. Overall, these results suggest that supplementation with threonine during colitis induction impact goblet cell number and delays the recovery period. This reinforces the importance of a deeper understanding regarding threonine supplementation in IBD.por
dc.description.sponsorshipNorthern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (FEDER) (NORTE-01-0145-FEDER-000013) and the Fundação para a Ciência e Tecnologia (FCT) (contracts PD/BD/106053/2015 to JG via Inter-University Doctoral Programme in Ageing and Chronic Disease – PhDOC, IF/00021/2014 to RS, IF/01390/2014 to ET, IF/01147/2013 to RD-O, IF/00735/2014 to AgC, SFRH/BPD/96176/2013 to CC, and SFRH/BPD/111100/2015 to LG). MS is a FCT investigator. The NMR data was acquired at CERMAX (Centro de Ressonância Magnética António Xavier) which is a member of the National NMR network with the support of Project LISBOA-01-0145-FEDER-007660por
dc.language.isoengpor
dc.publisherFrontiers Mediapor
dc.rightsopenAccesspor
dc.subjectIBDpor
dc.subjectThreoninepor
dc.subjectDSS-induced colitispor
dc.subjectGoblet cellspor
dc.subjectMetabolomicspor
dc.subjectIL-22por
dc.subjectMucinpor
dc.titleL-Threonine Supplementation During Colitis Onset Delays Disease Recoverypor
dc.typearticlepor
dc.peerreviewedyespor
dc.relation.publisherversionhttps://www.frontiersin.org/articles/10.3389/fphys.2018.01247/fullpor
oaire.citationStartPage1247por
oaire.citationIssueSEPpor
oaire.citationVolume9por
dc.identifier.doi10.3389/fphys.2018.01247por
dc.subject.fosCiências Médicas::Medicina Básicapor
dc.description.publicationversioninfo:eu-repo/semantics/publishedVersionpor
dc.subject.wosScience & Technologypor
sdum.journalFrontiers in Physiologypor
Aparece nas coleções:ICVS - Artigos em revistas internacionais / Papers in international journals

Ficheiros deste registo:
Ficheiro Descrição TamanhoFormato 
L-threonine supplementation during colitis onset delays disease recovery.pdf1,05 MBAdobe PDFVer/Abrir

Partilhe no FacebookPartilhe no TwitterPartilhe no DeliciousPartilhe no LinkedInPartilhe no DiggAdicionar ao Google BookmarksPartilhe no MySpacePartilhe no Orkut
Exporte no formato BibTex mendeley Exporte no formato Endnote Adicione ao seu ORCID