Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/58775
Título: | Chondrogenesis-inductive nanofibrous substrate using both biological fluids and mesenchymal stem cells from an autologous source |
Autor(es): | Casanova, Marta Alexandra Rodrigues Alves da Silva, M. Costa-Pinto, A. R. Reis, R. L. Martins, A. Neves, N. M. |
Palavras-chave: | Chondrogenic differentiation Electrospun nanofibrous meshes Insulin-like growth factor-I (IGF-I) Platelet lysates Transforming growth factor-β3 (TGF-β3) Transforming growth factor-beta 3 (TGF-beta 3) |
Data: | Mai-2019 |
Editora: | Elsevier |
Revista: | Materials Science and Engineering: C |
Citação: | Casanova M. R., Alves da Silva M., Costa-Pinto A. R., Reis R. L., Martins A., Neves N. M. Chondrogenesis-inductive nanofibrous substrate using both biological fluids and mesenchymal stem cells from an autologous source, Materials Science and Engineering: C, Vol. 98, pp. 1169-1178, doi:10.1016/j.msec.2019.01.069, 2019 |
Resumo(s): | During the last decade, many cartilage tissue engineering strategies have been developed, being the stem cell-based approach one of the most promising. Transforming Growth Factor-β3 (TGF-β3) and Insulin-like Growth Factor-I (IGF-I) are key proteins involved in the regulation of chondrogenic differentiation. Therefore, these two growth factors (GFs) were immobilized at the surface of a single electrospun nanofibrous mesh (NFM) aiming to differentiate human Bone Marrow-derived Mesenchymal Stem Cells (hBM-MSCs). The immobilization of defined antibodies (i.e. anti-TGF-β3 and anti-IGF-I) allows the selective retrieval of the abovementioned GFs from human platelet lysates (PL). Biochemical assays, involving hBM-MSCs cultured on biofunctional nanofibrous substrates under basal culture medium during 28⠯days, confirm the biological activity of bound TGF-β3 and IGF-I. Specifically, the typical spherical morphology of chondrocytes and the immunolocalization of collagen type II confirmed the formation of a cartilaginous ECM. Therefore, the proposed biofunctional nanofibrous substrate is able to promote chondrogenesis. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/58775 |
DOI: | 10.1016/j.msec.2019.01.069 |
ISSN: | 0928-4931 |
Versão da editora: | https://doi.org/10.1016/j.msec.2019.01.069. |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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19726-1-s2.0-S0928493118303382-main (1).pdf Acesso restrito! | 1,61 MB | Adobe PDF | Ver/Abrir |