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https://hdl.handle.net/1822/58941
Título: | p63 expression in solid cell nests of the thyroid: further evidence for a stem cell origin |
Autor(es): | Reis-Filho, Jorge S. Preto, Ana Soares, Paula Ricardo, Sara Cameselle-Teijeiro, José Sobrinho-Simões, Manuel |
Palavras-chave: | Adenoma Adult Aged Biomarkers, Tumor Carcinoembryonic Antigen Carcinoma, Papillary DNA-Binding Proteins Female Genes, Tumor Suppressor Humans Immunoenzyme Techniques Keratins Male Middle Aged Phosphoproteins Stem Cells Thyroid Neoplasms Trans-Activators Transcription Factors Tumor Suppressor Proteins Membrane Proteins p63 immunohistochemistry thyroid ultimobranchial body |
Data: | Jan-2003 |
Editora: | Nature Publishing Group |
Revista: | Modern Pathology |
Resumo(s): | Solid cell nests of the thyroid are embryonic remnants of endodermal origin that may be difficult to distinguish from squamous metaplasia, metastatic squamous carcinoma, papillary microcarcinoma, medullary carcinoma, and C-cell hyperplasia. These embryonic structures are composed of main cells and C-cells; cystic structures and mixed follicles are sometimes observed intermingled with solid cell nests. Recently, p63, a p53 homologue that is consistently expressed in basal/stem cells of stratified epithelia and plays a major role in triggering the differentiation of some specific cell lineages, has been characterized. We evaluated the immunohistochemical expression of p63, cytokeratins (CAM 5.2, AE1/AE3, 34betaE12, 7, and 20), carcinoembryonic antigen, thyroid transcription factor 1 (TTF-1), thyroglobulin, and calcitonin using the streptavidin-biotin-peroxidase complex technique in 6 bona fide solid cell nests. We observed that main cells of solid cell nests are strongly decorated by p63, while C-cells and all other thyroid structures were consistently negative. Moreover, main cells expressed carcinoembryonic antigen and all cytokeratins but cytokeratin 20 and lacked TTF-1, thyroglobulin and calcitonin. In contrast to this, C-cells of solid cell nests were immunoreactive for calcitonin, CAM 5.2, AE1/AE3, and cytokeratin 7; focal immunoreactivity for TTF-1 was also observed in some C-cells. We conclude that main cells of the solid cell nests display a basal/stem cell phenotype (p63 and basal cytokeratin positivity), whereas C-cells show features of parafollicular differentiation. We conclude, furthermore, that p63 antibodies may help in distinguishing solid cell nests from their mimics. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/58941 |
DOI: | 10.1097/01.MP.0000047306.72278.39 |
ISSN: | 0893-3952 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
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3880708.pdf Acesso restrito! | 810,51 kB | Adobe PDF | Ver/Abrir |