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https://hdl.handle.net/1822/60188
Título: | Light exposure during growth increases riboflavin production, reactive oxygen species accumulation and DNA damage in Ashbya gossypii riboflavin-overproducing strains |
Autor(es): | Silva, Rui Aguiar, Tatiana Quinta Oliveira, Rui Pedro Soares de Domingues, Lucília |
Palavras-chave: | Ashbya gossypii Riboflavin Comet assay Photosensitizer Light-induced DNA damage ROS |
Data: | 2019 |
Editora: | Oxford University Press |
Revista: | FEMS Yeast Research |
Citação: | Silva, Rui; Aguiar, Tatiana Q.; Oliveira, Rui; Domingues, Lucília, Light exposure during growth increases riboflavin production, reactive oxygen species accumulation and DNA damage in Ashbya gossypii riboflavin-overproducing strains. FEMS Yeast Research, 19(1), foy114, 2019 |
Resumo(s): | The overproduction of riboflavin (vitamin B2) by Ashbya gossypii, one of the most distinctive traits of this filamentous hemiascomycete, has been proposed to act as an ecological defense mechanism, since it is triggered by environmental stress. The interaction of endogenous riboflavin with light generates reactive oxygen species (ROS) and induces oxidative DNA damage in mammalian cells, but exogenous riboflavin was shown to protect A. gossypii spores against ultraviolet light. Envisioning a better understanding of this biotechnologically relevant trait, here we investigated the putative genotoxic effects associated with the overproduction of riboflavin by A. gossypii. For assessing that we developed the Ashbya Comet Assay, which was able to reproducibly measure oxidative (H2O2/menadione-mediated) and non-oxidative (camptothecin-mediated) DNA damage in A. gossypii. Using this protocol, we determined that exposure to sunlight-mimicking light during growth significantly increased the DNA damage accumulation in riboflavin-overproducing cells, but not in non-overproducing ones. The exposure of overproducing cells to light induced the intracellular accumulation of ROS and increased the production of riboflavin 1.5-fold. These results show that riboflavin-overproducing strains are highly susceptible to photo-induced oxidative DNA damage and draw attention for the importance of controlling the exposure to light of biotechnological riboflavin production processes with A. gossypii. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/60188 |
ISBN: | 1567-1364 |
DOI: | 10.1093/femsyr/foy114 |
ISSN: | 1567-1356 |
e-ISSN: | 1567-1364 |
Versão da editora: | http://femsyr.oxfordjournals.org/ |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_49216_1.pdf | 1,02 MB | Adobe PDF | Ver/Abrir |