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https://hdl.handle.net/1822/60331
Título: | LED optrode with integrated temperature sensing for optogenetics |
Autor(es): | Goncalves, S. Beatriz Palha, José Marinho Cruz Fernandes, Helena C. Souto, Márcio R. Pimenta, Sara Dong, Tao Yang, Zhaochu Ribeiro, J. F. Correia, J. H. |
Palavras-chave: | silicon neural probes LED chip thermoresistance temperature monitoring optogenetics |
Data: | 17-Set-2018 |
Editora: | Multidisciplinary Digital Publishing Institute |
Revista: | Micromachines |
Resumo(s): | In optogenetic studies, the brain is exposed to high-power light sources and inadequate power density or exposure time can cause cell damage from overheating (typically temperature increasing of 2 ∘ C). In order to overcome overheating issues in optogenetics, this paper presents a neural tool capable of assessing tissue temperature over time, combined with the capability of electrical recording and optical stimulation. A silicon-based 8 mm long probe was manufactured to reach deep neural structures. The final proof-of-concept device comprises a double-sided function: on one side, an optrode with LED-based stimulation and platinum (Pt) recording points; and, on the opposite side, a Pt-based thin-film thermoresistance (RTD) for temperature assessing in the photostimulation site surroundings. Pt thin-films for tissue interface were chosen due to its biocompatibility and thermal linearity. A single-shaft probe is demonstrated for integration in a 3D probe array. A 3D probe array will reduce the distance between the thermal sensor and the heating source. Results show good recording and optical features, with average impedance magnitude of 371 k Ω , at 1 kHz, and optical power of 1.2 mW·mm −2 (at 470 nm), respectively. The manufactured RTD showed resolution of 0.2 ∘ C at 37 ∘ C (normal body temperature). Overall, the results show a device capable of meeting the requirements of a neural interface for recording/stimulating of neural activity and monitoring temperature profile of the photostimulation site surroundings, which suggests a promising tool for neuroscience research filed. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/60331 |
DOI: | 10.3390/mi9090473 |
ISSN: | 2072-666X |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
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Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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micromachines-09-00473.pdf | 6,09 MB | Adobe PDF | Ver/Abrir |