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https://hdl.handle.net/1822/60440
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Campo DC | Valor | Idioma |
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dc.contributor.author | Guimarães, Diana Isabel Pereira | por |
dc.contributor.author | Noro, Jennifer Martins | por |
dc.contributor.author | Loureiro, Ana Isabel Sá | por |
dc.contributor.author | Cavaco-Paulo, Artur | por |
dc.contributor.author | Nogueira, Eugénia Sofia Costa | por |
dc.date.accessioned | 2019-05-29T13:43:30Z | - |
dc.date.available | 2019-05-29T13:43:30Z | - |
dc.date.issued | 2019-07 | - |
dc.date.submitted | 2018-12 | - |
dc.identifier.citation | Guimarães, D., Noro, J., Loureiro, A., Cavaco-Paulo, A., & Nogueira, E. (2019). Quantification of drugs encapsulated in liposomes by 1H NMR. Colloids and Surfaces B: Biointerfaces, 179, 414-420 | por |
dc.identifier.issn | 0927-7765 | por |
dc.identifier.uri | https://hdl.handle.net/1822/60440 | - |
dc.description.abstract | Liposomes are one of the most important and extensively studied drug delivery system due to their ability to encapsulate different kinds of drugs. Exploiting the advantages of 1H Nuclear Magnetic Resonance (NMR) spectrometry, we established a rapid and easy method for quantification of drugs encapsulated in liposomes. An internal standard, pyridine, was used for quantitative determination of drug concentration. Two different drugs were involved in this work, one hydrophilic, methotrexate disodium salt, and another hydrophobic, tamoxifen. The specificity and selectivity of the suggested method were evaluated by the absence of overlapping of at least one signal of each drug with pyridine in the NMR spectrum. The accuracy and precision of the method were assessed by adding a known amount of each drug to unloaded liposomes. Results obtained by quantitative NMR (qNMR) were validated and confirmed by comparing with two other traditional techniques, Ultraviolet - Visible (UV-Vis) spectrophotometry and High-Performance Liquid Chromatography (HPLC). It was found that the results were consistent with the ones obtained from our proposed qNMR method. Considering all the experiments conducted in this study, we deliberate that qNMR can be a suitable tool for the determination of drugs encapsulated in liposomes. | por |
dc.description.sponsorship | This work has received funding from the European Union Horizon 2020 research andinnovation programme undergrantagreement NMP06-2015-683356 FOLSMART. This study was also supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2013 unit and COMPETE 2020 (POCI-01-0145-FEDER-006684) and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte. Diana Guimarães (SFRH/BD/140321/ 2018) and Jennifer Noro (SFRH/BD/121673/2016) holds a scholarship from FCT. SFRH/BD/121673/2016 | por |
dc.language.iso | eng | por |
dc.publisher | Elsevier | por |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/683356/EU | por |
dc.relation | info:eu-repo/grantAgreement/FCT/5876/147337/PT | por |
dc.rights | openAccess | por |
dc.subject | Liposomes | por |
dc.subject | Drugs | por |
dc.subject | QuantificationH NMR | por |
dc.subject | UV-Vis | por |
dc.subject | HPLC-UV/Vis | por |
dc.subject | 1 H NMR | por |
dc.subject | Quantification | por |
dc.subject | H-1 NMR | por |
dc.title | Quantification of drugs encapsulated in liposomes by 1H NMR | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.journals.elsevier.com/colloids-and-surfaces-b-biointerfaces/ | por |
dc.comments | CEB51665 | por |
oaire.citationStartPage | 414 | por |
oaire.citationEndPage | 420 | por |
oaire.citationConferencePlace | Netherlands | - |
oaire.citationVolume | 179 | por |
dc.date.updated | 2019-04-27T09:54:35Z | - |
dc.identifier.eissn | 0927-7765 | por |
dc.identifier.doi | 10.1016/j.colsurfb.2019.03.039 | por |
dc.identifier.pmid | 30999120 | por |
dc.subject.fos | Engenharia e Tecnologia::Biotecnologia Industrial | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Colloids and Surfaces B: Biointerfaces | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_51665_1.pdf | 769,76 kB | Adobe PDF | Ver/Abrir |