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dc.contributor.authorPinheiro, T.por
dc.contributor.authorCoelho, Eduardo João Louropor
dc.contributor.authorRomani, Aloia Perezpor
dc.contributor.authorDomingues, Lucíliapor
dc.identifier.citationPinheiro, T.; Coelho, Eduardo; Romaní, Aloia; Domingues, Lucília, Intensifying ethanol production from brewers spent grain waste: use of whole slurry at high solid loadings. New Biotechnology, 53, 1-8, 2019por
dc.description.abstractThis work targets the valorization of brewers spent grain (BSG) waste by ethanol production, providing strategies for increasing titers in the multiple process steps involved. High solid loadings and use of whole slurry from the pretreatment were evaluated, aiming to achieve high ethanol concentration and yield. As variability in BSG chemical composition presents a challenge for their valorization, six different BSGs were chemically characterized, which allowed the selection of two with high polysaccharide content. High solid loadings (up to 25%) were employed for the pretreatment of selected BSGs by autohydrolysis, an environmentally friendly process, to improve enzymatic saccharification and extract fermentable sugars as oligosaccharides and monosaccharides. As a result, high glucose concentrations (43.7 and 57.7?g L-1) and glucose yield (85.9 and 70.6 %) were obtained from the saccharification of the pretreated BSG whole slurry at 20 and 25% solid loading, respectively. Whole slurries from autohydrolysis were used as substrate for ethanol production by hybrid saccharification and fermentation. Two different Saccharomyces cerevisiae strains were evaluated and high ethanol concentration (42.27?g L-1) at a high yield (94.0 %) was achieved. The results attained by the combined intensification approaches qualify BSG waste as a valuable renewable resource for cost-effective ethanol production.por
dc.description.sponsorshipThis study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of UID/BIO/04469/2019 unit and COMPETE 2020 (POCI-01-0145-FEDER006684), BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by European Regional Development Fund under the scope of Norte2020 - Programa Operacional Regional do Norte and MultiBiorefinery project (POCI-01-0145-FEDER-016403). Furthermore, authors acknowledge Fermentum-Engenharia das Fermentações Lda., for providing raw material for this work.por
dc.subjectBrewers spent grain wastepor
dc.subjectwhole slurrypor
dc.subjecthigh gravity ethanolpor
dc.subjectindustrial strainspor
dc.titleIntensifying ethanol production from brewers spent grain waste: use of whole slurry at high solid loadingspor
dc.subject.wosScience & Technologypor
sdum.journalNew Biotechnologypor
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