Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/62104
Título: | Gene disruption using zinc finger nuclease technology |
Autor(es): | Granja, Sara Costa Marchiq, Ibtissam Baltazar, Fátima Pouysségur, Jacques |
Palavras-chave: | Basigin Cell Line, Tumor Deoxyribonuclease I Flow Cytometry Gene Knockout Techniques Humans Plasmids Transfection Transformation, Genetic Zinc Fingers DNA double-strand breaks Gene targeting Knockout Zinc finger nuclease |
Data: | 2014 |
Editora: | Humana Press |
Revista: | Methods in Molecular Biology |
Resumo(s): | Zinc finger nucleases are reagents that induce DNA double-strand breaks at specific sites that can be repaired by nonhomologous end joining, inducing alterations in the genome. This strategy has enabled highly efficient gene disruption in numerous cell types and model organisms opening a door for new therapeutic applications. Here, we describe the disruption of CD147/basigin by this technique in a human cancer cell line. |
Tipo: | Capítulo de livro |
URI: | https://hdl.handle.net/1822/62104 |
ISBN: | 978-1-4939-0855-4 |
e-ISBN: | 978-1-4939-0856-1 |
DOI: | 10.1007/978-1-4939-0856-1_17 |
ISSN: | 1064-3745 |
e-ISSN: | 1940-6029 |
Acesso: | Acesso restrito autor |
Aparece nas coleções: |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
---|---|---|---|---|
granja2014.pdf Acesso restrito! | 151,31 kB | Adobe PDF | Ver/Abrir |