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TitleKnock out of the BASIGIN/CD147 chaperone of lactate/H+ symporters disproves its pro-tumour action via extracellular matrix metalloproteases (MMPs) induction
Author(s)Marchiq, Ibtissam
Albrengues, Jean
Granja, Sara Costa
Gaggioli, Cédric
Pouysségur, Jacques
Simon, Marie-Pierre
Cell Line, Tumor
Coculture Techniques
Culture Media, Conditioned
Gene Expression Regulation, Neoplastic
Matrix Metalloproteinases
Monocarboxylic Acid Transporters
Muscle Proteins
Paracrine Communication
Signal Transduction
Zinc Fingers
Gene Knockout Techniques
cancer biology
membrane transport
lactic acid
Issue date22-Sep-2015
PublisherImpact Journals
CitationMarchiq, I., Albrengues, J., Granja, S., Gaggioli, C., Pouysségur, J., & Simon, M. P. (2015). Knock out of the BASIGIN/CD147 chaperone of lactate/H+ symporters disproves its pro-tumour action via extracellular matrix metalloproteases (MMPs) induction. Oncotarget, 6(28), 24636.
Abstract(s)BASIGIN/CD147/EMMPRIN is a multifunctional transmembrane glycoprotein strongly expressed in tumours. BASIGIN controls tumour metabolism, particularly glycolysis by facilitating lactic acid export through the two monocarboxylate transporters MCT1 and hypoxia-inducible MCT4. However, before being recognized as a co-carrier of MCTs, BASIGIN was described as an inducer of extracellular matrix metalloproteases (MMPs). Early on, a model emerged in which, tumour cells use the extracellular domain of BASIGIN to recognize and stimulate neighbouring fibroblasts to produce MMPs. However, this model has remained hypothetical since a direct link between BASIGIN and MMPs production has not yet been clearly established. To validate the BASIGIN/MMP hypothesis, we developed BASIGIN knockouts in three human tumour cell lines derived from glioma, colon, and lung adenocarcinoma. By using co-culture experiments of either human or mouse fibroblasts and tumour cell lines we showed, contrary to what has been abundantly published, that the disruption of BASIGIN in tumour cells and in MEFs has no action on the production of MMPs. Our findings do not support the notion that the pro-tumoural action of BASIGIN is mediated via induction of MMPs. Therefore, we propose that to date, the strongest pro-tumoural action of BASIGIN is mediated through the control of fermentative glycolysis.
AccessOpen access
Appears in Collections:ICVS - Artigos em revistas internacionais / Papers in international journals

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