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dc.contributor.authorPereira-Leal, José Bpor
dc.contributor.authorAbreu, Isabel Apor
dc.contributor.authorAlabaça, Cláudia Spor
dc.contributor.authorAlmeida, Maria Helenapor
dc.contributor.authorAlmeida, Paulopor
dc.contributor.authorAlmeida, Tâniapor
dc.contributor.authorAmorim, Maria Isabelpor
dc.contributor.authorAraújo, Susanapor
dc.contributor.authorAzevedo, Herlânderpor
dc.contributor.authorBadia, Aleixpor
dc.contributor.authorBatista, Dorapor
dc.contributor.authorBohn, Andreaspor
dc.contributor.authorCapote, Tiagopor
dc.contributor.authorCarrasquinho, Isabelpor
dc.contributor.authorChaves, Inêspor
dc.contributor.authorCoelho, Ana Cristinapor
dc.contributor.authorCosta, Maria Manuela Ribeiropor
dc.contributor.authorCosta, Ritapor
dc.contributor.authorCravador, Alfredopor
dc.contributor.authorEgas, Conceiçãopor
dc.contributor.authorFaro, Carlospor
dc.contributor.authorFortes, Ana Mpor
dc.contributor.authorFortunato, Ana Spor
dc.contributor.authorGaspar, Maria Joãopor
dc.contributor.authorGonçalves, Sóniapor
dc.contributor.authorGraça, Josépor
dc.contributor.authorHorta, Maríliapor
dc.contributor.authorInácio, Verapor
dc.contributor.authorLeitão, José Mpor
dc.contributor.authorLino-Neto, T.por
dc.contributor.authorMarum, Lilianapor
dc.contributor.authorMatos, Josépor
dc.contributor.authorMendonça, Diogopor
dc.contributor.authorMiguel, Andreiapor
dc.contributor.authorMiguel, Célia Mpor
dc.contributor.authorMorais-Cecílio, Leonorpor
dc.contributor.authorNeves, Isabelpor
dc.contributor.authorNóbrega, Filomenapor
dc.contributor.authorOliveira, Maria Margaridapor
dc.contributor.authorOliveira, Rutepor
dc.contributor.authorPais, Maria Salomépor
dc.contributor.authorPaiva, Jorge Apor
dc.contributor.authorPaulo, Octávio Spor
dc.contributor.authorPinheiro, Miguelpor
dc.contributor.authorRaimundo, João A Ppor
dc.contributor.authorRamalho, José Cpor
dc.contributor.authorRibeiro, Ana Ipor
dc.contributor.authorRibeiro, Teresapor
dc.contributor.authorRocheta, Margaridapor
dc.contributor.authorRodrigues, Ana Isabelpor
dc.contributor.authorRodrigues, José Cpor
dc.contributor.authorSaibo, Nelson J Mpor
dc.contributor.authorSanto, Tatiana Epor
dc.contributor.authorSantos, Ana Margaridapor
dc.contributor.authorSá-Pereira, Paulapor
dc.contributor.authorSebastiana, Mónicapor
dc.contributor.authorSimões, Fernandapor
dc.contributor.authorSobral, Rómulo Spor
dc.contributor.authorTavares, Ruipor
dc.contributor.authorTeixeira, Ritapor
dc.contributor.authorVarela, Carolinapor
dc.contributor.authorVeloso, Maria Manuelapor
dc.contributor.authorRicardo, Cândido P. P.por
dc.description.abstractBackground: Cork oak (Quercus suber) is one of the rare trees with the ability to produce cork, a material widely used to make wine bottle stoppers, flooring and insulation materials, among many other uses. The molecular mechanisms of cork formation are still poorly understood, in great part due to the difficulty in studying a species with a long life-cycle and for which there is scarce molecular/genomic information. Cork oak forests are of great ecological importance and represent a major economic and social resource in Southern Europe and Northern Africa. However, global warming is threatening the cork oak forests by imposing thermal, hydric and many types of novel biotic stresses. Despite the economic and social value of the Q. suber species, few genomic resources have been developed, useful for biotechnological applications and improved forest management. Results: We generated in excess of 7 million sequence reads, by pyrosequencing 21 normalized cDNA libraries derived from multiple Q. suber tissues and organs, developmental stages and physiological conditions. We deployed a stringent sequence processing and assembly pipeline that resulted in the identification of ~159,000 unigenes. These were annotated according to their similarity to known plant genes, to known Interpro domains, GO classes and E.C. numbers. The phylogenetic extent of this ESTs set was investigated, and we found that cork oak revealed a significant new gene space that is not covered by other model species or EST sequencing projects. The raw data, as well as the full annotated assembly, are now available to the community in a dedicated web portal at Conclusions: This genomic resource represents the first trancriptome study in a cork producing species. It can be explored to develop new tools and approaches to understand stress responses and developmental processes in forest trees, as well as the molecular cascades underlying cork differentiation and disease response.eng
dc.description.sponsorshipThis project was funded by "Fundacao para a Ciencia e a Tecnologia" (FCT) within a National Consortium (COEC - Cork Oak ESTs Consortium) that supported 12 sub-projects (SOBREIRO/033, 035, 014, 034, 015, 017, 038, 019, 029, 039, 030, 036/2009). The authors further wish to acknowledge FCT for ten doctoral (BD) and post doctoral (BPD) fellowships (Tania Almeida: SFRH/BD/44410/2008, Tiago Capote: SFRH/BD/69785/2010, Ines Chaves: SFRH/BPD/20833/2004, Ana S. Fortunato: SFRH/BPD/47563/2008, Marilia Horta: SFRH/BPD/63213/2009, Liliana Marum: "SFRH/BPD/47679/2008, Andreia Miguel: SFRH/BD/44474/2008, Margarida Rocheta: SFRH/BPD/64905/2009, Tatiana E. Santo: SFRH/BD/47450/2008, Monica Sebastiana: SFRH/BPD/25661/2005). Andreas Bohn, Nelson J.M. Saibo, Rita Teixeira were supported by the Programa Ciencia 2007, financed by POPH (QREN) and Isabel A. Abreu, Susana Araujo, Dora Batista, A. Margarida Fortes, Jorge A.P. Paiva, Sonia Goncalves by Programa Ciencia 2008, also funded by POPH (QREN). A Margarida Santos was funded through iBET (PEst-OE/EQB/LA0004/2011). Maintenance of the CorkOakDB is supported by the Instituto Gulbenkian de Ciencia.por
dc.subjectDNA, Plantpor
dc.subjectGene Librarypor
dc.subjectSequence Analysis, DNApor
dc.subjectExpressed Sequence Tagspor
dc.titleA comprehensive assessment of the transcriptome of cork oak (Quercus suber) through EST sequencingpor
dc.subject.wosScience & Technologypor
sdum.journalBMC Genomicspor
Appears in Collections:CBFP - Artigos/Papers
DBio - Artigos/Papers

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