Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/64096

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dc.contributor.authorCosta, Diana Pereira Soarespor
dc.contributor.authorSousa, João Carlospor
dc.contributor.authorMesquita, Sandro Gabriel Ferreira Dápor
dc.contributor.authorPetkova, Nevena Ivanovapor
dc.contributor.authorMarques, Fernandapor
dc.contributor.authorReis, R. L.por
dc.contributor.authorSousa, Nunopor
dc.contributor.authorPashkuleva, I.por
dc.date.accessioned2020-02-26T16:55:45Z-
dc.date.available2020-02-26T16:55:45Z-
dc.date.issued2020-02-
dc.date.submitted2020-02-
dc.identifier.citationSoares da Costa D., Sousa J. C., da Mesquita S., Petkova-Yankova N., Marques F., Reis R. L., Sousa N., Pashkuleva I. Bioorthogonal Labeling Reveals Different Expression of Glycans in Mouse Hippocampal Neuron Cultures during Their Development, Molecules, Vol. 25, Issue 4, pp. 795, doi:10.3390/molecules25040795, 2020por
dc.identifier.issn1420-3049por
dc.identifier.urihttps://hdl.handle.net/1822/64096-
dc.descriptionThe following are available online. Scheme S1: Synthesis of peracetylated azidomannose (Ac4ManNAz); Scheme S2: Alternative synthesis of GlcNAz using chloroacetic anhydride and NaOH as a base; Scheme S3: Synthesis of peracetylated azidofucose (Ac4FucAz); Figure S1: 1H-NMR spectra of ManNAz (D2O, 300 MHz); Figure S2: 1H-NMR spectra of Ac4ManNAz (CDCl3 , 300 MHz), mixture of anomers; Figure S3: HPLC chromatogram of purified Ac4ManNAz showing the two anomers; Figure S4: 1H-NMR spectra of GlcNAz (D2O, 300MHz); Figure S5: 1H-NMR spectra of Ac4GlcNAz (CDCl3 , 300 MHz), mixture of anomers; Figure S6: HPLC chromatogram of purified Ac4GlcNAz showing the two anomers; Figure S7: 1H-NMR spectra of 6-azido-1,2,3,4-tetra-O-acetyl-6-deoxy-α,β-L-galactopyranose Ac4FucAz (CDCl3 , 300 MHz): mixture of anomers; Table S1: Primer sequences used in qRT-PCR.-
dc.description.abstractThe expression of different glycans at the cell surface dictates cell interactions with their environment and other cells, being crucial for the cell fate. The development of the central nervous system is associated with tremendous changes in the cell glycome that is tightly regulated. Herein, we have employed biorthogonal Cu-free click chemistry to image temporal distribution of different glycans in live mouse hippocampal neurons during their maturation in vitro. We show development-dependent glycan patterns with increased fucose and decreased mannose expression at the end of the maturation process. We also demonstrate that this approach is biocompatible and does not affect glycan transport although it relies on an administration of modified glycans. The applicability of this strategy to tissue sections unlocks new opportunities to study the glycan dynamics under more complex physiological conditions.por
dc.description.sponsorshipThis research was funded by the European Union Framework Programme for Research and Innovation Horizon 2020 under grant agreement n.º 668983—FoReCaST, by European Union’s Horizon 2020 Research and Innovation programme, under the Grant Agreement number 739572–The Discoveries CTR, and the project NORTE-01-0145-FEDER-000023, supported by the Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (FEDER).por
dc.language.isoengpor
dc.publisherMDPIpor
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/668983/EU-
dc.relationinfo:eu-repo/grantAgreement/EC/H2020/739572/EU-
dc.rightsopenAccesspor
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/-
dc.subjectBioorthogonal chemistrypor
dc.subjectglycosylationpor
dc.subjectImagingpor
dc.subjectNeuronal developmentpor
dc.subjectBiorthogonal chemistrypor
dc.titleBioorthogonal labeling reveals different expression of glycans in mouse hippocampal neuron cultures during their developmentpor
dc.typearticle-
dc.peerreviewedyespor
dc.relation.publisherversionhttps://www.mdpi.com/1420-3049/25/4/795por
dc.commentshttp://3bs.uminho.pt/node/20257por
oaire.citationIssue4por
oaire.citationVolume25por
dc.date.updated2020-02-26T16:23:56Z-
dc.identifier.doi10.3390/molecules25040795por
dc.identifier.pmid32059500por
dc.subject.wosScience & Technologypor
sdum.journalMoleculespor
oaire.versionVoR-
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals
ICVS - Artigos em revistas internacionais / Papers in international journals

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