Please use this identifier to cite or link to this item: https://hdl.handle.net/1822/64150

Title(+)-Limonene 1,2-Epoxide-Loaded SLNs: Evaluation of Drug Release, Antioxidant Activity, and Cytotoxicity in an HaCaT Cell Line
Author(s)Souto, Eliana B.
Zielinska, Aleksandra
Souto, Selma B.
Durazzo, Alessandra
Lucarini, Massimo
Santini, Antonello
Silva, Amélia M.
Atanasov, Atanas G.
Marques, Conrado
Andrade, Luciana N.
Severino, Patricia
Keywords(+)-limonene 1
2-epoxide
monoterpene
Imwitor 900K-SLN
lipid peroxidation
cytotoxicity
HaCaT cell line
(+)-limonene 1,2-epoxide
Issue date20-Feb-2020
PublisherMDPI
JournalInternational Journal of Molecular Sciences
CitationSouto, Eliana; Zielinska, Aleksandra; Souto, Selma B.; Durazzo, Alessandra; Lucarini, Massimo; Santini, Antonello; Silva, Amélia M.; Atanasov, Atanas G.; Marques, Conrado; Andrade, Luciana N.; Severino, Patricia, (+)-Limonene 1,2-Epoxide-Loaded SLNs: Evaluation of Drug Release, Antioxidant Activity, and Cytotoxicity in an HaCaT Cell Line. International Journal of Molecular Sciences, 21(4), 1449, 2020
Abstract(s)In this work, we developed a solid lipid nanoparticle (SLN) formulation with (+)-limonene 1,2-epoxide and glycerol monostearate (Lim-SLNs), stabilized with Poloxamer® 188 in aqueous dispersion to modify the release profile of the loaded monoterpene derivative. We also evaluated the role of SLNs in lipid peroxidation and cytotoxicity in a spontaneously transformed aneuploid immortal keratinocyte cell line from adult human skin (the HaCaT cell line). For the cell viability assay, the colorimetric 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used. Lim-SLNs with a loading capacity and encapsulation efficiency of 0.39% and 63%, respectively, were produced by high pressure homogenization. A mean particle size of 194 ± 3.4 nm and polydispersity index of 0.244 were recorded for the loaded Lim-SLNs, as compared to 203 ± 1.5 nm (PI 0.213) for the non-loaded (blank) SLNs. The loading of the monoterpene derivative into glycerol monostearate SLNs fitted into the zero-order kinetics, and ameliorated both lipid peroxidation and cytotoxicity in a keratinocyte cell line. A promising formulation for antioxidant and anti-tumoral activities is here proposed.
TypeArticle
URIhttps://hdl.handle.net/1822/64150
ISBN1424-6783
DOI10.3390/ijms21041449
ISSN1422-0067
e-ISSN1661-6596
Publisher versionhttp://www.mdpi.com/journal/ijms
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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