Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/64150
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Souto, Eliana B. | por |
dc.contributor.author | Zielinska, Aleksandra | por |
dc.contributor.author | Souto, Selma B. | por |
dc.contributor.author | Durazzo, Alessandra | por |
dc.contributor.author | Lucarini, Massimo | por |
dc.contributor.author | Santini, Antonello | por |
dc.contributor.author | Silva, Amélia M. | por |
dc.contributor.author | Atanasov, Atanas G. | por |
dc.contributor.author | Marques, Conrado | por |
dc.contributor.author | Andrade, Luciana N. | por |
dc.contributor.author | Severino, Patricia | por |
dc.date.accessioned | 2020-03-02T15:15:53Z | - |
dc.date.available | 2020-03-02T15:15:53Z | - |
dc.date.issued | 2020-02-20 | - |
dc.identifier.citation | Souto, Eliana; Zielinska, Aleksandra; Souto, Selma B.; Durazzo, Alessandra; Lucarini, Massimo; Santini, Antonello; Silva, Amélia M.; Atanasov, Atanas G.; Marques, Conrado; Andrade, Luciana N.; Severino, Patricia, (+)-Limonene 1,2-Epoxide-Loaded SLNs: Evaluation of Drug Release, Antioxidant Activity, and Cytotoxicity in an HaCaT Cell Line. International Journal of Molecular Sciences, 21(4), 1449, 2020 | por |
dc.identifier.isbn | 1424-6783 | - |
dc.identifier.issn | 1422-0067 | por |
dc.identifier.uri | https://hdl.handle.net/1822/64150 | - |
dc.description.abstract | In this work, we developed a solid lipid nanoparticle (SLN) formulation with (+)-limonene 1,2-epoxide and glycerol monostearate (Lim-SLNs), stabilized with Poloxamer® 188 in aqueous dispersion to modify the release profile of the loaded monoterpene derivative. We also evaluated the role of SLNs in lipid peroxidation and cytotoxicity in a spontaneously transformed aneuploid immortal keratinocyte cell line from adult human skin (the HaCaT cell line). For the cell viability assay, the colorimetric 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay was used. Lim-SLNs with a loading capacity and encapsulation efficiency of 0.39% and 63%, respectively, were produced by high pressure homogenization. A mean particle size of 194 ± 3.4 nm and polydispersity index of 0.244 were recorded for the loaded Lim-SLNs, as compared to 203 ± 1.5 nm (PI 0.213) for the non-loaded (blank) SLNs. The loading of the monoterpene derivative into glycerol monostearate SLNs fitted into the zero-order kinetics, and ameliorated both lipid peroxidation and cytotoxicity in a keratinocyte cell line. A promising formulation for antioxidant and anti-tumoral activities is here proposed. | por |
dc.description.sponsorship | The authors wish to acknowledge the financial support from CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico, from CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior), and from FAPITEC/SE (Fundação de Apoio à Pesquisa e Inovação Tecnológica do Estado de Sergipe). E. B. Souto acknowledges the sponsorship of the projects M-ERA-NET-0004/2015-PAIRED and UIDB/04469/2020 (strategic fund), received support from the Portuguese Science and Technology Foundation, Ministry of Science and Education (FCT/MEC) through national funds, and was co-financed by FEDER, under the Partnership Agreement PT2020. A.M. Silva acknowledges FCT/MEC for the project PEst-/UID/AGR/04033/2019 (CITAB). | por |
dc.language.iso | eng | por |
dc.publisher | MDPI | por |
dc.relation | UIDB/04469/2020 | por |
dc.relation | PEst-/UID/AGR/04033/2019 | por |
dc.rights | openAccess | por |
dc.subject | (+)-limonene 1 | por |
dc.subject | 2-epoxide | por |
dc.subject | monoterpene | por |
dc.subject | Imwitor 900K-SLN | por |
dc.subject | lipid peroxidation | por |
dc.subject | cytotoxicity | por |
dc.subject | HaCaT cell line | por |
dc.subject | (+)-limonene 1,2-epoxide | por |
dc.title | (+)-Limonene 1,2-Epoxide-Loaded SLNs: Evaluation of Drug Release, Antioxidant Activity, and Cytotoxicity in an HaCaT Cell Line | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | http://www.mdpi.com/journal/ijms | por |
dc.comments | CEB53510 | por |
oaire.citationIssue | 4 | por |
oaire.citationVolume | 21 | por |
dc.date.updated | 2020-02-22T20:43:23Z | - |
dc.identifier.eissn | 1661-6596 | por |
dc.identifier.doi | 10.3390/ijms21041449 | por |
dc.identifier.pmid | 32093358 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | International Journal of Molecular Sciences | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_53510_1.pdf | 1,19 MB | Adobe PDF | Ver/Abrir |