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TitleCombination of a peptide-modified gellan gum hydrogel with cell therapy in a lumbar spinal cord injury animal model
Author(s)Gomes, Eduardo D.
Mendes, Sofia Silva
Leite-Almeida, Hugo
Gimble, Jeffrey M.
Tam, Roger Y.
Shoichet, Molly S.
Sousa, Nuno
Silva, Nuno André Martins
Salgado, A. J.
Combined Modality Therapy
Lumbar Vertebrae
Nerve Regeneration
Polysaccharides, Bacterial
Rats, Wistar
Spinal Cord Injuries
Stem Cell Transplantation
Treatment Outcome
Regenerative medicine
Adipose tissue-derived Stromal/Stem
Cells Olfactory Ensheathing Cells
Extracellular matrix like hydrogels
Spinal cord injury
Adipose tissue-derived Stromal/Stem Cells
Olfactory Ensheathing Cells
Issue date2016
CitationGomes, E. D., Mendes, S. S., Leite-Almeida, H., Gimble, J. M., et. al. (2016). Combination of a peptide-modified gellan gum hydrogel with cell therapy in a lumbar spinal cord injury animal model. Biomaterials, 105, 38-51.
Abstract(s)Spinal Cord Injury (SCI) is a highly incapacitating condition for which there is still no cure. Current clinical approaches are mainly based on palliative care, so there is a need to find possible treatments to SCI. Cellular transplantation is regarded with great expectation due to the therapeutic potential of cells such as Adipose tissue-derived Stromal/Stem Cells (ASCs) or Olfactory Ensheathing Cells (OECs). Both are accessible sources and present positive paracrine and cell-to-cell interactions, previously reported by our group. Additionally, biomaterials such as hydrogels have been applied in SCI repair with promising results. We propose to combine a GRGDS-modified gellan gum hydrogel with ASCs and OECs in order to promote SCI regeneration. In vitro, ASCs and OECs could be co-cultured within GG-GRGDS hydrogels inducing a more robust neurite outgrowth when compared to controls. In vivo experiments in a hemisection SCI rat model revealed that the administration of ASCs and OECs encapsulated in a GG-GRGDS hydrogel led to significant motor improvements when compared to both control (SCI) and hydrogel alone (GG-GRGDS) groups. This was accompanied by a decreased infiltration of inflammatory cells and astrocytes, and by an increased intensity of neurofilament. These results suggest evident gains induced by the encapsulation of ASCs and OECs in GG-GRGDS based hydrogels.
Publisher version
AccessRestricted access (UMinho)
Appears in Collections:ICVS - Artigos em revistas internacionais / Papers in international journals

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