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Campo DCValorIdioma
dc.contributor.authorGomes, Eduardo D.por
dc.contributor.authorMendes, Sofia Silvapor
dc.contributor.authorLeite-Almeida, Hugopor
dc.contributor.authorGimble, Jeffrey M.por
dc.contributor.authorTam, Roger Y.por
dc.contributor.authorShoichet, Molly S.por
dc.contributor.authorSousa, Nunopor
dc.contributor.authorSilva, Nuno André Martinspor
dc.contributor.authorSalgado, A. J.por
dc.date.accessioned2020-03-06T12:17:36Z-
dc.date.issued2016-
dc.identifier.citationGomes, E. D., Mendes, S. S., Leite-Almeida, H., Gimble, J. M., et. al. (2016). Combination of a peptide-modified gellan gum hydrogel with cell therapy in a lumbar spinal cord injury animal model. Biomaterials, 105, 38-51.por
dc.identifier.issn0142-9612-
dc.identifier.urihttps://hdl.handle.net/1822/64296-
dc.description.abstractSpinal Cord Injury (SCI) is a highly incapacitating condition for which there is still no cure. Current clinical approaches are mainly based on palliative care, so there is a need to find possible treatments to SCI. Cellular transplantation is regarded with great expectation due to the therapeutic potential of cells such as Adipose tissue-derived Stromal/Stem Cells (ASCs) or Olfactory Ensheathing Cells (OECs). Both are accessible sources and present positive paracrine and cell-to-cell interactions, previously reported by our group. Additionally, biomaterials such as hydrogels have been applied in SCI repair with promising results. We propose to combine a GRGDS-modified gellan gum hydrogel with ASCs and OECs in order to promote SCI regeneration. In vitro, ASCs and OECs could be co-cultured within GG-GRGDS hydrogels inducing a more robust neurite outgrowth when compared to controls. In vivo experiments in a hemisection SCI rat model revealed that the administration of ASCs and OECs encapsulated in a GG-GRGDS hydrogel led to significant motor improvements when compared to both control (SCI) and hydrogel alone (GG-GRGDS) groups. This was accompanied by a decreased infiltration of inflammatory cells and astrocytes, and by an increased intensity of neurofilament. These results suggest evident gains induced by the encapsulation of ASCs and OECs in GG-GRGDS based hydrogels.por
dc.description.sponsorshipPortuguese Foundation for Science and Technology [Doctoral fellowship (SFRH/BD/103075/2014) to E. D. Gomes; IF Development Grant to A. J. Salgado; Post-Doctoral fellowship (SFRH/BPD/97701/2013) to N. A. Silva]; This article has been developed under the scope of the projects NORTE-01-0145-FEDER-000013, supported by the Northern Portugal Regional Operational Programme (NORTE 2020), under the Portugal 2020 Partnership Agreement, through the European Regional Development Fund (FEDER); This work has been funded by FEDER funds, through the Competitiveness Factors Operational Programme (COMPETE), and by National funds, through the Foundation for Science and Technology (FCT), under the scope of the project POCI-01-0145-FEDER-007038por
dc.language.isoengpor
dc.publisherElsevier-
dc.rightsrestrictedAccesspor
dc.subjectAnimalspor
dc.subjectCombined Modality Therapypor
dc.subjectHydrogelspor
dc.subjectLumbar Vertebraepor
dc.subjectNerve Regenerationpor
dc.subjectOligopeptidespor
dc.subjectPolysaccharides, Bacterialpor
dc.subjectRatspor
dc.subjectRats, Wistarpor
dc.subjectSpinal Cord Injuriespor
dc.subjectStem Cell Transplantationpor
dc.subjectTreatment Outcomepor
dc.subjectRegenerative medicinepor
dc.subjectAdipose tissue-derived Stromal/Stempor
dc.subjectCells Olfactory Ensheathing Cellspor
dc.subjectExtracellular matrix like hydrogelspor
dc.subjectSpinal cord injurypor
dc.subjectAdipose tissue-derived Stromal/Stem Cellspor
dc.subjectOlfactory Ensheathing Cellspor
dc.titleCombination of a peptide-modified gellan gum hydrogel with cell therapy in a lumbar spinal cord injury animal modelpor
dc.typearticlepor
dc.peerreviewedyespor
dc.relation.publisherversionhttps://www.sciencedirect.com/science/article/pii/S0142961216303507por
oaire.citationStartPage38por
oaire.citationEndPage51por
oaire.citationVolume105por
dc.identifier.eissn1878-5905-
dc.identifier.doi10.1016/j.biomaterials.2016.07.019por
dc.date.embargo10000-01-01-
dc.identifier.pmid27505621por
dc.subject.fosCiências Médicas::Medicina Básicapor
dc.subject.wosScience & Technologypor
sdum.journalBiomaterialspor
Aparece nas coleções:ICVS - Artigos em revistas internacionais / Papers in international journals

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