Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/64506

TítuloExploring the gelation mechanisms and cytocompatibility of gold (III)-mediated regenerated and thiolated silk fibroin hydrogels
Autor(es)Laomeephol, Chavee
Ferreira, Helena Susana Costa Machado
Yodmuang, Supansa
Reis, R. L.
Damrongsakkul, Siriporn
Neves, N. M.
Palavras-chaveCytocompatibility
Gold
hydrogel
Silk Fibroin
thiolated silk fibroin
DataMar-2020
EditoraMDPI AG
RevistaBiomolecules
CitaçãoLaomeephol C., Ferreira H., Yodmuang S., Reis R. L., Damrongsakkul S., Neves N. M. Exploring the Gelation Mechanisms and Cytocompatibility of Gold (III)-Mediated Regenerated and Thiolated Silk Fibroin Hydrogels, Biomolecules, Vol. 10, Issue 3, pp. 1-14, doi:10.3390/biom10030466, 2020
Resumo(s)Accelerating the gelation of silk fibroin (SF) solution from several days or weeks to minutes or few hours is critical for several applications (e.g., cell encapsulation, bio-ink for 3D printing, and injectable controlled release). In this study, the rapid gelation of SF induced by a gold salt (Au3+) as well as the cytocompatibility of Au3+-mediated SF hydrogels are reported. The gelation behaviors and mechanisms of regenerated SF and thiolated SF (tSF) were compared. Hydrogels can be obtained immediately after mixing or within three days depending on the types of silk proteins used and amount of Au3+. Au3+-mediated SF and tSF hydrogels showed different color appearances. The color of Au-SF hydrogels was purple-red, whereas the Au-tSF hydrogels maintained their initial solution color, indicating different gelation mechanisms. The reduction of Au3+ by amino groups and further reduction to Au by tyrosine present in SF, resulting in a dityrosine bonding and Au nanoparticles (NPs) production, are proposed as underlying mechanisms of Au-SF gel formation. Thiol groups of the tSF reduced Au3+ to Au+ and formed a disulfide bond, before a formation of Au+-S bonds. Protons generated during the reactions between Au3+ and SF or tSF led to a decrease of the local pH, which affected the chain aggregation of the SF, and induced the conformational transition of SF protein to beta sheet. The cytocompatibility of the Au-SF and tSF hydrogels was demonstrated by culturing with a L929 cell line, indicating that the developed hydrogels can be promising 3D matrices for different biomedical applications.
TipoArtigo
URIhttps://hdl.handle.net/1822/64506
DOI10.3390/biom10030466
e-ISSN2218-273X
Versão da editorahttps://www.mdpi.com/2218-273X/10/3/466
Arbitragem científicayes
AcessoAcesso aberto
Aparece nas coleções:3B’s - Artigos em revistas/Papers in scientific journals

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