Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/65506

TítuloMicrobial metabolic potential of phenol degradation in wastewater treatment plant of crude oil refinery: analysis of metagenomes and characterization of isolates
Autor(es)Viggor, Signe
Jõesaar, Merike
Soares-Castro, Pedro
Ilmjärv, Tanel
Santos, P. M.
Kapley, Atya
Kivisaar, Maia
Palavras-chavephenol
bacterial community
oil refinery wastewater
metagenome
Pseudomonas
Acinetobacter
Data30-Abr-2020
EditoraMultidisciplinary Digital Publishing Institute
RevistaMicroorganisms
CitaçãoViggor, S.; Jõesaar, M.; Soares-Castro, P.; Ilmjärv, T.; Santos, P.M.; Kapley, A.; Kivisaar, M. Microbial Metabolic Potential of Phenol Degradation in Wastewater Treatment Plant of Crude Oil Refinery: Analysis of Metagenomes and Characterization of Isolates. Microorganisms 2020, 8, 652.
Resumo(s)The drilling, processing and transportation of oil are the main sources of pollution in water and soil. The current work analyzes the microbial diversity and aromatic compounds degradation potential in the metagenomes of communities in the wastewater treatment plant (WWTP) of a crude oil refinery. By focusing on the degradation of phenol, we observed the involvement of diverse indigenous microbial communities at different steps of the WWTP. The anaerobic bacterial and archaeal genera were replaced by aerobic and facultative anaerobic bacteria through the biological treatment processes. The phyla Proteobacteria, Bacteroidetes and Planctomycetes were dominating at different stages of the treatment. Most of the established protein sequences of the phenol degradation key enzymes belonged to bacteria from the class Alphaproteobacteria. From 35 isolated strains, 14 were able to grow on aromatic compounds, whereas several phenolic compound-degrading strains also degraded aliphatic hydrocarbons. Two strains, <i>Acinetobacter venetianus</i> ICP1 and <i>Pseudomonas oleovorans</i> ICTN13, were able to degrade various aromatic and aliphatic pollutants and were further characterized by whole genome sequencing and cultivation experiments in the presence of phenol to ascertain their metabolic capacity in phenol degradation. When grown alone, the intermediates of catechol degradation, the meta or ortho pathways, accumulated into the growth environment of these strains. In the mixed cultures of the strains ICP1 and ICTN13, phenol was degraded via cooperation, in which the strain ICP1 was responsible for the adherence of cells and ICTN13 diminished the accumulation of toxic intermediates.
TipoArtigo
URIhttps://hdl.handle.net/1822/65506
DOI10.3390/microorganisms8050652
Versão da editorahttps://www.mdpi.com/2076-2607/8/5/652
Arbitragem científicayes
AcessoAcesso aberto
Aparece nas coleções:CBMA - Artigos/Papers
DBio - Artigos/Papers

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