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https://hdl.handle.net/1822/65633
Título: | Thymus zygis subsp. zygis an endemic portuguese plant: phytochemical profiling, antioxidant, anti-proliferative and anti-inflammatory activities |
Autor(es): | Silva, Amélia M. Martins-Gomes, Carlos Souto, Eliana B. Schäfer, Judith Santos, João A. Bunzel, Mirko Nunes, Fernando M. |
Palavras-chave: | Thymus zygis subsp. zygis phenolic profiling aqueous decoction hydroethanolic extract luteolin-O-hexoside anti-proliferative activity radical scavenging activity antioxidant anti-inflammatory activity Thymus zygis subsp Zygis |
Data: | 3-Jun-2020 |
Editora: | MDPI |
Revista: | Antioxidants |
Citação: | Silva, Amélia M.; Martins-Gomes, Carlos; Souto, Eliana; Schäfer, Judith; Santos, João A.; Bunzel, Mirko; Nunes, Fernando M., Thymus zygis subsp. zygis an endemic portuguese plant: phytochemical profiling, antioxidant, anti-proliferative and anti-inflammatory activities. Antioxidants, 9(6), 482, 2020 |
Resumo(s): | Thymus zygis subsp. zygis is an endemic Portuguese plant belonging to the Thymus zygis species. Although T. zygis is commonly used as a condiment and as a medicinal herb, a detailed description of the polyphenol composition of hydroethanolic (HE) and aqueous decoction (AD) extracts is not available. In this work, we describe for the first time a detailed phenolic composition of Thymus zygis subsp. zygis HE and AD extracts, together with their antioxidant, anti-proliferative and anti-inflammatory activities. Unlike other Thymus species, T. zygis subsp. zygis extracts contain higher amounts of luteolin-(?)-O-hexoside. However, the major phenolic compound is rosmarinic acid, and high amounts of salvianolic acids K and I were also detected. T. zygis subsp. zygis extracts exhibited significant scavenging activity of ABTS+, hydroxyl (•OH), and nitric oxide (NO) radicals. Regarding the anti-proliferative/cytotoxic effect, tested against Caco-2 and HepG2 cells, the AD extract only slightly reduced cell viability at higher concentrations (IC50 > 600 µg/mL, 48 h exposure), denoting very low toxicity, while the HE extract showed a high anti-proliferative effect, especially at 48 h exposure (IC50 of 85.01 ± 15.10 μg/mL and 82.19 ± 2.46 μg/mL, for Caco-2 and HepG2, respectively). At non-cytotoxic concentrations, both extracts reduced the nitric oxide (NO) release by lipopolysaccharide (LPS)-stimulated RAW 264.7 cells (at 50 μg/mL, HE and AD extracts inhibited NO release in ~89% and 48%, respectively). In conclusion, the results highlight the non-toxic effect of aqueous extracts, both resembling the consumption of antioxidants in foodstuff or in functional food. Furthermore, the HE extract of T. zygis subsp. zygis is a source of promising molecules with antioxidant, anti-inflammatory and anticancer activities, highlighting its potential as a source of bioactive ingredients for nutraceutical and pharmaceutical industries. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/65633 |
DOI: | 10.3390/antiox9060482 |
e-ISSN: | 2076-3921 |
Versão da editora: | https://www.mdpi.com/journal/antioxidants |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_53753_1.pdf | 1,89 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons