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https://hdl.handle.net/1822/65650
Título: | Optimization of nimesulide-loaded solid lipid nanoparticles (SLN) by factorial design, release profile and cytotoxicity in human Colon adenocarcinoma cell line |
Autor(es): | Campos, Joana R. Fernandes, Ana R. Sousa, Raquel Fangueiro, Joana F. Boonme, Prapaporn Garcia, Maria Luisa Silva, Amelia M. Naveros, Beatriz C. Souto, Eliana B. |
Palavras-chave: | Nimesulide solid lipid nanoparticles physical stability factorial design experiment lyophilization trehalose cryoprotectants |
Data: | Mai-2019 |
Editora: | Marcel Dekker Inc. |
Revista: | Pharmaceutical Development and Technology |
Citação: | Campos, Joana R.; Fernandes, Ana R.; Sousa, Raquel; Fangueiro, Joana F.; Boonme, Prapaporn; Garcia, Maria Luisa; Silva, Amelia M.; Naveros, Beatriz C.; Souto, Eliana, Optimization of nimesulide-loaded solid lipid nanoparticles (SLN) by factorial design, release profile and cytotoxicity in human Colon adenocarcinoma cell line. Pharmaceutical Development and Technology, 24(5), 616-622, 2019 |
Resumo(s): | The aim of this work is development of a nontoxic, long-term stable solid lipid nanoparticles (SLN) formulation for the loading of Nimesulide (NiM) by a 22 factorial design. The optimized formulation was composed of 10wt% of glyceryl behenate and 2.5wt% of poloxamer 188. Immediately after production, Z-Ave of NiM-SLN was 166.1±0.114nm, with a polydispersity index (PI) of 0.171±0051 and zeta potential nearly neutral (3.10±0.166mV). A slight increase of Z-Ave was recorded for NiM-SLN stored at 25°C for a period of 15days, whereas at 4°C particles kept size within similar range. Long-term stability was monitored using TurbiscanLab®, showing a high stability of the nanoparticles with variations in the backscattering profiles below 10%. The release profile of NiM-SLN followed a sustained pattern with ca. 30% of drug released up to 24h. Empty-SLN and NiM-SLN were nontoxic after exposing Caco-2 cells to the highest concentration (100g/mL) up to 48hours (cell viability higher than 80%). NiM-SLN were lyophilized using different cryoprotectants, producing particles of 463.1±36.63nm (PI 0.491±0.027) with 5% trehalose. Solid character of NiM-SLN was confirmed by DSC, recording a recrystallization index of 83% for NiM-SLN and of 74% for lyophilized SLN. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/65650 |
DOI: | 10.1080/10837450.2018.1549075 |
ISSN: | 1083-7450 |
e-ISSN: | 1097-9867 |
Versão da editora: | https://www.tandfonline.com/loi/iphd20 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_49486_1.pdf | 563,34 kB | Adobe PDF | Ver/Abrir |