Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/66189
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Campo DC | Valor | Idioma |
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dc.contributor.author | Dencheva, Nadya | por |
dc.contributor.author | Braz, Joana | por |
dc.contributor.author | Scheibel, Dieter | por |
dc.contributor.author | Malfois, Marc | por |
dc.contributor.author | Denchev, Z. | por |
dc.contributor.author | Gitsov, Ivan | por |
dc.date.accessioned | 2020-07-30T14:18:17Z | - |
dc.date.available | 2020-07-30T14:18:17Z | - |
dc.date.issued | 2020 | - |
dc.identifier.citation | Dencheva, N.; Braz, J.; Scheibel, D.; Malfois, M.; Denchev, Z.; Gitsov, I. Polymer-Assisted Biocatalysis: Polyamide 4 Microparticles as Promising Carriers of Enzymatic Function. Catalysts 2020, 10, 767. | por |
dc.identifier.uri | https://hdl.handle.net/1822/66189 | - |
dc.description.abstract | This study reports a new strategy for enzyme immobilization based on passive immobilization in neat and magnetically responsive polyamide 4 (PA4) highly porous particles. The microsized particulate supports were synthesized by low-temperature activated anionic ring-opening polymerization. The enzyme of choice was laccase from <i>Trametes versicolor</i> and was immobilized by either adsorption on prefabricated PA4 microparticles (PA4@iL) or by physical in situ entrapment during the PA4 synthesis (PA4@eL). The surface topography of all PA4 particulate supports and laccase conjugates, as well as their chemical and physical structure, were studied by microscopic, spectral, thermal, and synchrotron WAXS/SAXS methods. The laccase content and activity in each conjugate were determined by complementary spectral and enzyme activity measurements. PA4@eL samples displayed >93% enzyme retention after five incubation cycles in an aqueous medium, and the PA4@iL series retained ca. 60% of the laccase. The newly synthesized PA4-laccase complexes were successfully used in dyestuff decolorization aiming at potential applications in effluent remediation. All of them displayed excellent decolorization of positively charged dyestuffs reaching ~100% in 15 min. With negative dyes after 24 h the decolorization reached 55% for PA4@iL and 85% for PA4@eL. A second consecutive decolorization test revealed only a 5–10% decrease in effectiveness indicating the reusability potential of the laccase-PA4 conjugates. | por |
dc.description.sponsorship | The authors gratefully acknowledge the financial support of the project TSSiPRONORTE-01-0145-FEDER-000015, supported by the regional operation program NORTE2020, under the Portugal2020 Partnership Agreement, through the European Regional Development Fund, as well as the support byNational Funds throughFundação para a Ciência e Tecnologia(FCT), project UID/CTM/50025/2019. D.S. and I.G.wish to thank the Research Foundation of the State of New York for partial funding through Materials Network ofExcellence. N.D. is also grateful for the personal program-contract CTTI-51/18-IPC. | por |
dc.language.iso | eng | por |
dc.publisher | Multidisciplinary Digital Publishing Institute | por |
dc.relation | UID/CTM/50025/2019 | por |
dc.rights | openAccess | por |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | por |
dc.subject | laccase | por |
dc.subject | polyamide 4 | por |
dc.subject | enzyme immobilization | por |
dc.subject | magnetic enzyme supports | por |
dc.subject | enzyme decolorization | por |
dc.title | Polymer-assisted biocatalysis: polyamide 4 microparticles as promising carriers of enzymatic function | por |
dc.type | article | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://www.mdpi.com/2073-4344/10/7/767 | por |
oaire.citationStartPage | 1 | por |
oaire.citationEndPage | 29 | por |
oaire.citationIssue | 7 | por |
oaire.citationVolume | 10 | por |
dc.date.updated | 2020-07-24T13:37:20Z | - |
dc.identifier.eissn | 2073-4344 | - |
dc.identifier.doi | 10.3390/catal10070767 | por |
dc.subject.wos | Science & Technology | por |
sdum.journal | Catalysts | por |
oaire.version | VoR | por |
Aparece nas coleções: | IPC - Artigos em revistas científicas internacionais com arbitragem |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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catalysts-10-00767-v3.pdf | 6,5 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons