Please use this identifier to cite or link to this item: https://hdl.handle.net/1822/67053

TitleCroton argyrophyllus Kunth essential oil-loaded solid lipid nanoparticles: evaluation of release profile, antioxidant activity and cytotoxicity in a neuroblastoma cell line
Author(s)Souto, Eliana B.
Severino, Patricia
Marques, Conrado
Andrade, Luciana N.
Durazzo, Alessandra
Lucarini, Massimo
Atanasov, Atanas G.
El Maimouni, Soukaina
Novellino, Ettore
Santini, Antonello
Keywordsantioxidant
cytotoxic
essential oil
Croton argyrophyllus Kunth
neurological disorders
Issue date2020
PublisherMDPI
JournalSustainability (MDPI)
CitationSouto, Eliana; Severino, Patricia; Marques, Conrado; Andrade, Luciana N.; Durazzo, Alessandra; Lucarini, Massimo; Atanasov, Atanas G.; El Maimouni, Soukaina; Novellino, Ettore; Santini, Antonello, Croton argyrophyllus Kunth essential oil-loaded solid lipid nanoparticles: evaluation of release profile, antioxidant activity and cytotoxicity in a neuroblastoma cell line. Sustainability, 12(18), 7697, 2020
Abstract(s)The essential oil from Croton argyrophyllus Kunth is known for its antiproliferative, anti-inflammatory, antinociceptive, and anticancer activities, and is recognized as a source of phytochemicals for potential use in pharmaceutic and food sectors. Solid lipid nanoparticles (SLN) have been produced to load Croton argyrophyllus (CA) Kunth essential oil (CAEO) and its antioxidant properties evaluated in vitro as a new approach for the treatment of neurodegenerative diseases. Cetyl palmitate SLN loading CAEO (CAEO-SLN) with a mean particle size of 201.4 ± 2.3 nm (polydispersity index 0.211) have been produced by hot high-pressure homogenisation. The release of the oil followed the Korsmeyers-Peppas model. The risk of lipid peroxidation has been determined by applying the production of thiobarbituric acid-reactive substances (TBARS) standard assay. The antioxidant activity was determined by the capacity of the antioxidants existing in CAEO to scavenge the stable radical DPPH•. The cytotoxicity of CA Kunth essential oil-loaded SLN (CAEO-SLN) was evaluated in a human cell line SH-SY5Y (derived from human neuroblastoma) by determining the reduction of the yellow dye 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide (MTT). Both free essential oil (fEO) and loaded essential oil (CAEO-SLN) were demonstrated to inhibit the Fenton reaction. CAEO-SLN showed DPPH• radical scavenging capacity. The loading of the oil into cetyl palmitate SLN reduced the risk of cytotoxicity.
TypeArticle
URIhttps://hdl.handle.net/1822/67053
DOI10.3390/su12187697
ISSN2071-1050
Publisher versionhttps://www.mdpi.com/journal/sustainability
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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