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https://hdl.handle.net/1822/67542
Título: | The conserved and divergent roles of carbonic anhydrases in the filamentous fungi Aspergillus fumigatus and Aspergillus nidulans |
Autor(es): | Han, Kap-Hoon Chun, Yoon-Hee Figueiredo, Bárbara de Castro Pimentel Soriani, Frederico Marianetti Savoldi, Marcela Almeida, Agostinho João Ramalho Rodrigues, Fernando José dos Santos Cairns, Charlie Timothy Bignell, Elaine Tobal, Jaqueline Moisés Goldman, Maria Helena S. Kim, Jong-Hwan Bahn, Yong-Sun Goldman, Gustavo Henrique Ferreira, Márcia Eliana da Silva |
Palavras-chave: | Amino Acid Sequence Animals Aspergillosis Aspergillus fumigatus Aspergillus nidulans Carbon Dioxide Carbonic Anhydrases Cluster Analysis DNA, Fungal Disease Models, Animal Fungal Proteins Gene Deletion Gene Expression Profiling Genetic Complementation Test Mice Molecular Sequence Data Phylogeny Saccharomyces cerevisiae Sequence Alignment Sequence Homology Spores, Fungal Survival Analysis Virulence |
Data: | Mar-2010 |
Editora: | Wiley |
Revista: | Molecular Microbiology |
Citação: | Han, K. H., Chun, Y. H., De Castro Pimentel Figueiredo, B., Soriani, F. M., Savoldi, M., Almeida, A., ... & Goldman, M. H. S. (2010). The conserved and divergent roles of carbonic anhydrases in the filamentous fungi Aspergillus fumigatus and Aspergillus nidulans. Molecular microbiology, 75(6), 1372-1388 |
Resumo(s): | Carbon dioxide (CO(2)) and its hydration product bicarbonate (HCO(3)(-)) are essential molecules in various physiological processes of all living organisms. The reversible interconversion between CO(2) and HCO(3)(-) is in equilibrium. This reaction is slow without catalyst, but can be rapidly facilitated by Zn(2+)-metalloenzymes named carbonic anhydrases (CAs). To gain an insight into the function of multiple clades of fungal CA, we chose to investigate the filamentous fungi Aspergillus fumigatus and A. nidulans. We identified four and two CAs in A. fumigatus and A. nidulans, respectively, named cafA-D and canA-B. The cafA and cafB genes are constitutively, strongly expressed whereas cafC and cafD genes are weakly expressed but CO(2)-inducible. Heterologous expression of the A. fumigatus cafB, and A. nidulans canA and canB genes completely rescued the high CO(2)-requiring phenotype of a Saccharomyces cerevisiae Deltance103 mutant. Only the DeltacafA DeltacafB and DeltacanB deletion mutants were unable to grow at 0.033% CO(2), of which growth defects can be restored by high CO(2). Defects in the CAs can affect Aspergilli conidiation. Furthermore, A. fumigatus DeltacafA, DeltacafB, DeltacafC, DeltacafD and DeltacafA DeltacafB mutant strains are fully virulent in a low-dose murine infection. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/67542 |
DOI: | 10.1111/j.1365-2958.2010.07058.x |
ISSN: | 0950-382X |
e-ISSN: | 1365-2958 |
Versão da editora: | https://onlinelibrary.wiley.com/doi/full/10.1111/j.1365-2958.2010.07058.x |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Han-2010-The-conserved-and-divergent-roles-o.pdf Acesso restrito! | 1,23 MB | Adobe PDF | Ver/Abrir |