Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/67698
Título: | Computer-assisted immunohistochemical analysis of cervical cancer biomarkers using low-cost and simple software |
Autor(es): | Hammes, Luciano Serpa Korte, Jeffrey E. Tekmal, Rajeshwar Rao Naud, Paulo Edelweiss, Maria Isabel Valente, Philip T. Longatto, Adhemar Kirma, Nameer Cunha-Filho, João Sabino |
Palavras-chave: | Biomarkers, Tumor Coloring Agents Female Humans Image Processing, Computer-Assisted Uterine Cervical Neoplasms Immunohistochemistry Software Image analysis Cervical cancer biomarker computer-assisted |
Data: | Dez-2007 |
Editora: | Lippincott, Williams & Wilkins |
Revista: | Applied Immunohistochemistry & Molecular Morphology |
Resumo(s): | The study of biomarkers by immunohistochemistry (IHC) for cervical cancer and intraepithelial lesions is a promising field. However, manual interpretation of IHC and reproducibility of the scoring systems can be highly subjective. In this article, we present a novel and simple computer-assisted IHC interpretation method based on cyan-magenta-yellow-black (CMYK) color format, for tissues with diaminobenzidine cytoplasmatic staining counterstained with methyl green. This novel method is more easily interpreted than previous computer-assisted methods based on red-green-blue (RGB) color format and presents a strong correlation with the manual H-score. It is simple, objective, and requires only low-cost software and minimal computer skills. Briefly, a total of 67 microscopic images of cervical carcinoma, normal cervix, and negative controls were analyzed in Corel Photo Paint X3 software in CMYK and RGB color format, and compared with manual H-score IHC assessments. The clearest and best positive correlation with the H-score was obtained using the image in CMYK color format and crude values of magenta color (Spearman correlation coefficient=0.84; agreement of 93.33%, P<0.001). To obtain this value, only 3 steps were necessary: convert the image to CMYK format, select the area of interest for analysis, and open the color histogram tool to visualize the magenta value. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/67698 |
DOI: | 10.1097/01.pai.0000213151.64310.70 |
ISSN: | 1541-2016 |
e-ISSN: | 1533-4058 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito autor |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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hammes2007.pdf Acesso restrito! | 312,28 kB | Adobe PDF | Ver/Abrir |