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https://hdl.handle.net/1822/67937
Título: | IL-6 is constitutively expressed during lung morphogenesis and enhances fetal lung explant branching |
Autor(es): | Silva, Cristina Isabel Nogueira Santos, Marta Baptista, Maria J. Moura, Rute S. Correia-Pinto, Jorge |
Palavras-chave: | Animals Female Fetus Gene Expression Regulation, Developmental In Situ Hybridization Interleukin-6 MAP Kinase Signaling System Mitogen-Activated Protein Kinases Random Allocation Rats Rats, Sprague-Dawley Lung Morphogenesis |
Data: | Nov-2006 |
Editora: | Springer Nature |
Revista: | Pediatric Research |
Citação: | Nogueira-Silva, C., Santos, M., Baptista, M. J., Moura, R. S., & Correia-Pinto, J. (2006). IL-6 is constitutively expressed during lung morphogenesis and enhances fetal lung explant branching. Pediatric research, 60(5), 530-536 |
Resumo(s): | Previous studies have shown that chorioamnionitis, with increased IL-6, promotes fetal lung maturation and decreases the incidence of respiratory distress syndrome in premature neonates. However, the expression pattern and the effects of IL-6 on fetal lung growth mechanisms remain unknown. IL-6 expression was assessed by in situ hybridization and by real-time PCR between 14.5 and 21.5 d postconception. Normal and nitrofen-induced hypoplastic lung explants were cultured with increasing IL-6 doses or IL-6 neutralizing antibodies. Branching, cellular proliferation (Ki-67) and MAPK phosphorylation in fetal lung explants were analyzed. Pulmonary primitive epithelium expressed IL-6 constitutively throughout all gestational ages, displaying highest levels during earliest stages. In normal and hypoplastic lung explants, IL-6 neutralizing antibodies significantly reduced, whereas IL-6 supplementation induced a biphasic effect (lower doses increased, while the highest dose did not accomplish additional effect) on branching and cellular proliferation. IL-6 enhanced p38-MAPK phosphorylation without changing MEK1/2 and JNK pathways. The present study suggests a physiological role for IL-6 on pulmonary branching mechanisms most likely involving p38-MAPK intracellular signalling pathway. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/67937 |
DOI: | 10.1203/01.pdr.0000242300.09427.3b |
ISSN: | 0031-3998 |
e-ISSN: | 1530-0447 |
Versão da editora: | https://www.nature.com/articles/pr2006343 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | ICVS - Artigos em revistas internacionais / Papers in international journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Nogueira-silva-2006-Il--is-constitutively-expressed-dur.pdf | 1,14 MB | Adobe PDF | Ver/Abrir |