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https://hdl.handle.net/1822/68484
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Campo DC | Valor | Idioma |
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dc.contributor.author | Amorim, Cláudia | por |
dc.contributor.author | Braga, Adelaide | por |
dc.contributor.author | Rodrigues, Joana Lúcia Lima Correia | por |
dc.contributor.author | Cardoso, Beatriz A. Batista | por |
dc.contributor.author | Rainha, João | por |
dc.contributor.author | Gudiña, Eduardo José | por |
dc.contributor.author | Silvério, Sara Isabel Cruz | por |
dc.contributor.author | Rodrigues, L. R. | por |
dc.date.accessioned | 2020-12-10T15:39:10Z | - |
dc.date.available | 2020-12-10T15:39:10Z | - |
dc.date.issued | 2020-11-26 | - |
dc.identifier.citation | Amorim, Cláudia; Braga, Adelaide; Rodrigues, Joana L.; Cardoso, B.; , J. Rainha; Gudiña, Eduardo J.; Silvério, Sara C.; Rodrigues, Lígia R., Recombinant Saccharomyces cerevisiae as a microbial biocatalyst for the one-step production of prebiotic fructooligosaccharides. Biocatalysis Open Day. online, Nov 26, 2020. | por |
dc.identifier.uri | https://hdl.handle.net/1822/68484 | - |
dc.description.abstract | Fructooligosaccharides (FOS) are widely consumed prebiotics with proven beneficial effects on both human and animal health. As a result, alternative production processes with high-efficiency have been an increasing focus of interest by both academy and industry. In this work, a in vivo bioprocess approach was successfully developed for one-step production of FOS from sucrose fermentation by recombinant yeast. Saccharomyces cerevisiae YIL162W lacking the gene responsible for sucrose hydrolysis (suc2) was transformed to express the -fructofuranosidase (Ffase) INV gene from Schwanniomyces occidentalis (clone L196), and its mutated version containing a serine instead of a leucine at position 196 (clone S196), under the inducible GAL1 promotor. Clone S196 presented a 2.75-fold higher sucrolytic activity (22±3 U.mL-1), while clone L196 presented a higher efficiency towards FOS production, producing mainly 6-kestose (76±3 g.L-1) and 1-kestose (1.6±0.6 g.L- 1) after 24 h of fermentation at 30 °C and 200 rpm, in a medium containing 300 g/L of sucrose. Attending the potential of process simplification and cost-reduction, the Ffase INV gene was then expressed under the glyceraldehyde-3-phosphate dehydrogenase (GPD) constitutive promoter (clone GPD L196), resulting in a maximum FOS production of 61±4 g.L-1 ( 56±3 g.L-1 of 6-kestose and 5±31 of fructosylnystose) after 48 h of fermentation using 300 g/L of sucrose. Interestingly, the total amount of undesired glucose and fructose present in the media whenever the maximal FOS production was achieved, was 9 times lower with the GDP promoter (5.5±0.9 g.L-1). The present work demonstrates the high potential of this bioprocess approach for industrial production of prebiotic FOS in a single step. Nevertheless, there is still room for yield improvement in future work, namely through bioprocess optimization. | por |
dc.description.sponsorship | FCT under the scope of the strategic funding of UID/BIO/04469/2019 unit and the project FoSynBio (POCI-01-0145-FEDER-029549). The authors also acknowledge BioTecNorte operation (NORTE-01- 0145-FEDER-000004) and the project MultiBiorefinery (POCI-01-0145-FEDER-016403) funded by the European Regional Development Fund under the scope of Norte2020 – Programa Operacional Regional do Norte | por |
dc.language.iso | eng | por |
dc.rights | openAccess | por |
dc.title | Recombinant Saccharomyces cerevisiae as a microbial biocatalyst for the one-step production of prebiotic fructooligosaccharides | por |
dc.type | conferencePoster | por |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | www.efbiotechnology.org/biocatalysis_day | por |
dc.comments | CEB54032 | por |
dc.date.updated | 2020-12-08T14:16:32Z | - |
dc.subject.fos | Ciências Agrárias::Biotecnologia Agrária e Alimentar | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
sdum.conferencePublication | Biocatalysis Open Day | por |
Aparece nas coleções: | CEB - Painéis em Conferências / Posters in Conferences |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_54032_1.pdf | 170,4 kB | Adobe PDF | Ver/Abrir | |
document_54032_2.pdf | 778,39 kB | Adobe PDF | Ver/Abrir |