Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/69425
Título: | Liposomes embedded in layer by layer constructs as simplistic extracellular vesicles transfer model |
Autor(es): | Domínguez-Arca, V. Costa, R. R. Carvalho, Ana Margarida Moura Pereira Ferreira Taboada, P. Reis, R. L. Prieto, G. Pashkuleva, I. |
Palavras-chave: | Cell communication Extracellular matrix Glycosaminoglycans Layer-by-layer Liposomes Supported vesicle layers |
Data: | 2021 |
Editora: | Elsevier |
Revista: | Materials Science and Engineering: C |
Citação: | Domínguez-Arca, V., Costa, R. R., Carvalho, A. M., Taboada, P., Leis, R. R., Prieto, G., & Pashkuleva, I. (2021). Liposomes embedded in layer by layer constructs as simplistic extracellular vesicles transfer model. Materials Science and Engineering: C, 111813 |
Resumo(s): | Extracellular vesicles (EVs) are particles originating from the exfoliation of the cellular membrane. They are involved in cell-to-cell and cell-to-matrix signaling, exchange of bioactive molecules, tumorigenesis and metastasis, among others. To mitigate the limited understanding of EVs transfer phenomena, we developed a simplistic model that mimics EVs and their interactions with cells and the extracellular matrix. The proposed model is a layer-by-layer (LbL) film built from the polycationic poly-L-lysine (PLL) and the glycosaminoglycan hyaluronic acid (HA) to provide ECM mimicry. Positively charged 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and N1,N1,N14,N14-tetramethyl-N1,N14-ditetradecyltetradecane-1,14-diaminium dibromide (GS14) liposomes were embedded in this construct to act as EVs analogs. To simulate EVs carrying substances, Nile Red was loaded as a model of lipophilic cargo molecules. The integration of each component was followed by quartz-crystal microbalance measurements, which confirmed the immobilization of intact liposomes on the underlying (PLL/HA)3 soft film. The release of Nile Red from liposomes either embedded in the LbL construct or exposed at its surface revealed a fast first order release. This system was validated as a model for EV/cell interactions by incubation with breast cancer cells MDA-MB-231. We observed higher internalization for embedded liposomes when compared with surface-exposed ones, showcasing that the ECM mimic layers do not constitute a barrier to liposome/cell interactions but favor them. |
Tipo: | Artigo |
Descrição: | Journal Pre-proof |
URI: | https://hdl.handle.net/1822/69425 |
DOI: | 10.1016/j.msec.2020.111813 |
ISSN: | 0928-4931 |
Versão da editora: | https://www.sciencedirect.com/science/article/pii/S0928493120337322 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | 3B’s - Artigos em revistas/Papers in scientific journals |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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20438-1-s2.0-S0928493120337322-main.pdf Acesso restrito! | 1,39 MB | Adobe PDF | Ver/Abrir |