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TitleLipid-based nanostructures as a strategy to enhance curcumin bioaccessibility: behavior under digestion and cytotoxicity assessment
Author(s)Gonçalves, Raquel Filipa Silva
Martins, Joana Teresa Rodrigues
Abrunhosa, Luís
Baixinho, João
Matias, Ana A.
Vicente, A. A.
Pinheiro, A. C.
Nanostructured lipid carriers
Solid lipid nanoparticles
static digestion
Intestinal permeability
Lipid physical state
In vitro static digestion
Issue date2021
JournalFood Research International
CitationGonçalves, Raquel F. S.; Martins, Joana T.; Abrunhosa, Luís; Baixinho, João; Matias, Ana A.; Vicente, António A.; Pinheiro, Ana Cristina, Lipid-based nanostructures as a strategy to enhance curcumin bioaccessibility: behavior under digestion and cytotoxicity assessment. Food Research International, 143(110278), 2021
Abstract(s)The aim of this study was to evaluate the behavior of different lipid-based nanostructures during in vitro digestion, in particular on curcumins bioaccessibility, and to access their potential toxicity. Solid lipid nanoparticles (SLN), nanostructured lipid carriers (NLC) and nanoemulsions (NE) were submitted to harmonized static in vitro digestion and their cytotoxicity and cellular transport were evaluated using Caco-2 cell line. NE presented the highest curcumins bioaccessibility followed by NLC and SLN, 71.1%, 63.7% and 53.3%, respectively. Free fatty acids percentage increased in the following order: NLC ? NE < SLN. Non-digested nanostructures and excipients presented no cytotoxicity; however, digested NE and NLC presented cytotoxicity due to MCT oil, which presented cytotoxicity after digestion. The apparent permeability coefficient of NLC was higher than SLN and NE. These results showed that lipid-based nanostructures physical state and composition have a high influence on particles' behavior during digestion, and on their cytotoxicity/intestinal permeability, and highlights the importance of conducting cytotoxicity assessments after in vitro digestion. This work contributes to a better understanding of the behavior of lipid-based nanostructures under digestion/adsorption, and this knowledge will be useful in design of nanostructures that afford both safety and an increased bioactive compounds bioavailability.
Publisher version
AccessOpen access
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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