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TitleSurface functionalization of zeolite-based drug delivery systems enhances their antitumoral activity in vivo
Author(s)Vilaça, Natália
Bertão, Ana Raquel
Prasetyanto, Eko Adi
Granja, Sara
Costa, Marta
Fernandes, Rui
Figueiredo, Francisco
Fonseca, António
Cola, Luisa De
Baltazar, Fátima
Neves, Isabel C.
KeywordsZeolite nanoparticles
drug delivery systems (DDS)
Surface functionalization
cancer models
Chick Chorioallantoic Membrane (CAM) assay
Issue date2021
JournalMaterials Science and Engineering: C Materials for Biological Applications
CitationVilaça, Natália; Bertão, Ana Raquel; Prasetyanto, Eko Adi; Granja, Sara; Costa, Marta; Fernandes, Rui; Figueiredo, Francisco; Fonseca, António; Cola, Luisa De; Baltazar, Fátima; Neves, Isabel C., Surface functionalization of zeolite-based drug delivery systems enhances their antitumoral activity in vivo. Materials Science and Engineering C-Materials for Biological Applications, 120(111721), 2021.
Abstract(s)Zeolites have attractive features making them suitable carriers for drug delivery systems (DDS). As such, we loaded the anticancer drug 5-fluorouracil (5-FU), into two different zeolite structures, faujasite (NaY) and Linde Type L (LTL), to obtain different DDS. The prepared DDS were tested in vitro using breast cancer, colorectal carcinoma, and melanoma cell lines and in vivo using the chick embryo chorioallantoic membrane model (CAM). Both assays showed the best results for the Hs578T breast cancer cells, with a higher potentiation for 5-FU encapsulated in the zeolite LTL. To unveil the endocytic mechanisms involved in the internalization of the zeolite nanoparticles, endocytosis was inhibited pharmacologically in breast cancer and epithelial mammary human cells. The results suggest that a caveolin-mediated process was responsible for the internalized zeolite nanoparticles. Aiming to boost the DDS efficacy, the disc-shaped zeolite LTL outer surface was functionalized using amino (NH2) or carboxylic acid (COOH) groups and coated with poly-L-lysine (PLL). Positively functionalized surface LTL nanoparticles revealed to be non-toxic to human cells and, importantly, their internalization was faster and led to a higher tumor reduction in vivo. Overall, our results provide further insights into the mechanisms of interaction between zeolite-based DDS and cancer cells, and pave the way for future studies aiming to improve DDS anticancer activity.
DescriptionSupplementary data to this article can be found online at https://doi. org/10.1016/j.msec.2020.111721.
Publisher version
AccessRestricted access (UMinho)
Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series
ICVS - Artigos em revistas internacionais / Papers in international journals
CDQuim - Artigos (Papers)

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