Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/72508
Título: | Magnetically responsive PA6 microparticles with immobilized laccase show high catalytic efficiency in the enzymatic treatment of catechol |
Autor(es): | Dencheva, Nadya Vasileva Oliveira, Sandra Cristina Gomes Braz, Joana Filipa Barros Getya, Dariya Malfois, Marc Denchev, Z. Gitsov, Ivan |
Palavras-chave: | Laccase Polyamide 6 Enzyme immobilization Magnetic enzyme supports Catechol oxidation |
Data: | 11-Fev-2021 |
Editora: | Multidisciplinary Digital Publishing Institute (MDPI) |
Revista: | Catalysts |
Citação: | Dencheva, N.; Oliveira, S.; Braz, J.; Getya, D.; Malfois, M.; Denchev, Z.; Gitsov, I. Magnetically Responsive PA6 Microparticles with Immobilized Laccase Show High Catalytic Efficiency in the Enzymatic Treatment of Catechol. Catalysts 2021, 11, 239. https://doi.org/10.3390/catal11020239 |
Resumo(s): | Herewith we report the first attempt towards non-covalent immobilization of <i>Trametes versicolor</i> laccase on neat and magnetically responsive highly porous polyamide 6 (PA6) microparticles and their application for catechol oxidation. Four polyamide supports, namely neat PA6 and such carrying Fe, phosphate-coated Fe and Fe<sub>3</sub>O<sub>4</sub> cores were synthesized in suspension by activated anionic ring-opening polymerization (AAROP) of ε-caprolactam (ECL). Enzyme adsorption efficiency up to 92% was achieved in the immobilization process. All empty supports and PA6 laccase complexes were characterized by spectral and synchrotron WAXS/SAXS analyses. The activity of the immobilized laccase was evaluated using 2,2’-Azino-bis-(3- ethylbenzothiazoline-6-sulfonic acid (ABTS) and compared to the native enzyme. The PA6 laccase conjugates displayed up to 105% relative activity at room temperature, pH 4, 40 °C and 20 mM ionic strength (citrate buffer). The kinetic parameters of the ABTS oxidation were also determined. The reusability of the immobilized laccase-conjugates was proven for five consecutive oxidation cycles of catechol. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/72508 |
DOI: | 10.3390/catal11020239 |
e-ISSN: | 2073-4344 |
Versão da editora: | https://www.mdpi.com/2073-4344/11/2/239 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
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Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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catalysts-11-00239-v2.pdf | 6,38 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons