Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/72926
Título: | Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellates |
Autor(es): | Morais, Stephanie L. Barros, Piedade Santos, Marlene Delerue-Matos, Cristina Gomes, Andreia C Fátima Barroso, M. |
Palavras-chave: | DNA Probes Electrodes Gold Nucleic Acid Hybridization Biosensing Techniques Dinoflagellida Alexandrium minutum Chronoamperometry Dinoflagellate Electrochemical genosensor Sandwich format hybridization |
Data: | 15-Jan-2021 |
Editora: | Elsevier |
Revista: | Talanta |
Citação: | Morais, S. L., Barros, P., Santos, M., Delerue-Matos, C., et al. (2021). Electrochemical genosensor for the detection of Alexandrium minutum dinoflagellates. Talanta, 222, 121416 |
Resumo(s): | This work addresses the development of a disposable electrochemical genosensor for the detection of the toxic dinoflagellate, Alexandrium minutum. Analyzing public databases, a specific 70 bp DNA probe, targeting A. minutum, was selected and designed. The genosensor methodology implied the immobilization of a A. minutum-specific DNA-capture probe onto screen-printed gold electrodes (SPGE). To improve both the selectivity and to avoid strong secondary structures, that could hinder the hybridization efficiency, a sandwich format of the A. minutum gene was designed using a fluorescein isothiocyanate-labelled signaling DNA probe and enzymatic amplification of the electrochemical signal. Using this electrochemical genosensor, a concentration range from 0.12 to 1.0 nM, a LD of 24.78 pM with a RSD <5.2% was determined. The genosensor was successfully applied to the selective analysis of the targeted A. minutum specific region denatured genomic DNA extracted from toxic dinoflagellates present in the Atlantic Ocean. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/72926 |
DOI: | 10.1016/j.talanta.2020.121416 |
ISSN: | 0039-9140 |
Versão da editora: | https://www.sciencedirect.com/science/article/pii/S0039914020307074 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito autor |
Aparece nas coleções: | CBMA - Artigos/Papers DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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1-s2.0-S0039914020307074-main.pdf Acesso restrito! | 4,88 MB | Adobe PDF | Ver/Abrir |