Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/72966
Título: | A novel D-xylose isomerase from the gut of the wood feeding beetle Odontotaenius disjunctus efficiently expressed in Saccharomyces cerevisiae |
Autor(es): | Silva, Paulo César Ceja-Navarro, Javier A Azevedo, Flávio Karaoz, Ulas Brodie, Eoin L Johansson, Björn |
Data: | 2021 |
Editora: | Nature Research |
Revista: | Scientific Reports |
Citação: | Silva, Paulo César, Javier A. Ceja-Navarro, Flávio Azevedo, Ulas Karaoz, Eoin L. Brodie, and Björn Johansson. 2021. “A Novel D -Xylose Isomerase from the Gut of the Wood Feeding Beetle Odontotaenius Disjunctus Efficiently Expressed in Saccharomyces Cerevisiae.” Scientific Reports 11 (1): 1–12. |
Resumo(s): | Carbohydrate rich substrates such as lignocellulosic hydrolysates remain one of the primary sources of potentially renewable fuel and bulk chemicals. The pentose sugar D-xylose is often present in significant amounts along with hexoses. Saccharomyces cerevisiae can acquire the ability to metabolize D-xylose through expression of heterologous D-xylose isomerase (XI). This enzyme is notoriously difficult to express in S. cerevisiae and only fourteen XIs have been reported to be active so far. We cloned a new D-xylose isomerase derived from microorganisms in the gut of the wood-feeding beetle Odontotaenius disjunctus. Although somewhat homologous to the XI from Piromyces sp. E2, the new gene was identified as bacterial in origin and the host as a Parabacteroides sp. Expression of the new XI in S. cerevisiae resulted in faster aerobic growth than the XI from Piromyces on D-xylose media. The D-xylose isomerization rate conferred by the new XI was also 72% higher, while absolute xylitol production was identical in both strains. Interestingly, increasing concentrations of xylitol (up to 8 g L-1) appeared not to inhibit D-xylose consumption. The newly described XI displayed 2.6 times higher specific activity, 37% lower KM for D-xylose, and exhibited higher activity over a broader temperature range, retaining 51% of maximal activity at 30 °C compared with only 29% activity for the Piromyces XI. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/72966 |
DOI: | 10.1038/s41598-021-83937-z |
ISSN: | 2045-2322 |
Versão da editora: | https://www.nature.com/articles/s41598-021-83937-z |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CBMA - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Silva2021.pdf | 1,87 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons