Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/73004

TítuloCan parasites adapt to pollutants? A multigenerational experiment with a Daphnia x Metschnikowia model system exposed to the fungicide tebuconazole
Autor(es)Cuco, Ana P.
Wolinska, Justyna
Santos, Joana I.
Abrantes, Nelson
Goncalves, Fernando J. M.
Castro, Bruno B.
Palavras-chaveExperimental evolution
Host-parasite interactions
Multigenerational effects
Non-target aquatic fungi
Phenotypic plasticity
Data1-Set-2020
EditoraElsevier
RevistaAquatic Toxicology
Resumo(s)There is increasing evidence about negative effects of fungicides on non-target organisms, including parasitic species, which are key elements in food webs. Previous experiments showed that environmentally relevant concentrations of fungicide tebuconazole are toxic to the microparasite Metschnikowia bicuspidata, a yeast species that infects the planktonic crustacean Daphnia spp. However, due to their short-term nature, this and other experimental studies were not able to test if parasites could potentially adapt to these contaminants. Here, we tested if M. bicuspidata parasite can adapt to tebuconazole selective pressure. Infected D. magna lineages were reared under control conditions (no tebuconazole) and environmentally realistic tebuconazole concentrations, for four generations, and their performance was compared in a follow-up reciprocal assay. Additionally, we assessed whether the observed effects were transient (phenotypic) or permanent (genetic), by reassessing parasite fitness after the removal of selective pressure. Parasite fitness was negatively affected throughout the multigenerational exposure to the fungicide: prevalence of infection and spore load decreased, whereas host longevity increased, in comparison to control (naive) parasite lineages. In a follow-up reciprocal assay, tebuconazole-conditioned (TEB) lineages performed worse than naive parasite lineages, both in treatments without and with tebuconazole, confirming the cumulative negative effect of tebuconazole. The underperformance of TEB lineages was rapidly reversed after removing the influence of the selective pressure (tebuconazole), demonstrating that the costs of prolonged exposure to tebuconazole were phenotypic and transient. The microparasitic yeast M. bicuspidata did not reveal potential for rapid evolution to an anthropogenic selective pressure; instead, the long-term exposure to tebuconazole was hazardous to this non-target species. These findings highlight the potential environmental risks of azole fungicides on non-target parasit
TipoArtigo
URIhttps://hdl.handle.net/1822/73004
DOI10.1016/j.aquatox.2020.105584
ISSN0166-445X
32795838
Versão da editorahttps://www.journals.elsevier.com/aquatic-toxicology/
Arbitragem científicayes
AcessoAcesso aberto
Aparece nas coleções:CBMA - Artigos/Papers
DBio - Artigos/Papers

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