Please use this identifier to cite or link to this item: https://hdl.handle.net/1822/73092

TitleExploring the function and structure of the acetate transporter family
Author(s)Azevedo-Silva, João
Ribas, David
Castagnoli, Davide
Sa-Pessoa, Joana
Athayde, Diogo
Soares-Silva, Isabel
Archer, Margarida
Casal, Margarida
Issue date20-May-2018
PublisherElsevier
JournalFree Radical Biology and Medicine
CitationAzevedo-Silva, J., Ribas, D., Castagnoli, D., Sá-Pessoa, J., Athayde, D., et al.(2018). Exploring the function and structure of the acetate transporter family. Free Radical Biology and Medicine, 120, S118
Abstract(s)The AceTr transporter family is a group of Acetate Transporters with six predicted transmembrane segments. It has homologues in fungi, bacteria, archaea and protozoa. As acetate transporters, these proteins play a crucial role on cell metabolism being involved in the capacity of cells to adapt to nutrient availability. As these membrane transporters also transport other substrates, they may be an important asset for biotechnological purposes. To better understand the mechanism of acetate transport, we have investigated functional and structural features of the AceTr family. We have performed site-directed mutagenesis in residues highly conserved, or possibly involved in substrate binding, from the Saccharomyces cerevisiae Ady2. Functional assays determined the influence of these residues on the transport capacity and protein localization. Another goal of this work is to determine the structure of the AceTr family. Accordingly, several of its members were cloned in different expression vectors and their expression was evaluated in different E. coli expression strains. SatP from E. coli and its AceP from Methanosarcina acetivorans presented satisfactory levels of protein production and are currently being tested in production, purification and crystallization trials.
TypeAbstract
URIhttps://hdl.handle.net/1822/73092
DOI10.1016/j.freeradbiomed.2018.04.389
ISSN0891-5849
Publisher versionhttps://www.sciencedirect.com/science/article/pii/S0891584918305549
Peer-Reviewedyes
AccessRestricted access (UMinho)
Appears in Collections:CBMA - Resumos em livros de atas/Abstracts in proceedings

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