Please use this identifier to cite or link to this item: https://hdl.handle.net/1822/73134

TitleScreening the olive tree phyllosphere: Search and find potential antagonists against Pseudomonas savastanoi pv. savastanoi
Author(s)Mina, Diogo
Pereira, Jose Alberto
Lino-Neto, T.
Baptista, Paula
KeywordsOlea europaea
olive knot
biocontrol
Bacillus amyloliquefaciens
antagonistic mechanisms
Issue date2020
PublisherFrontiers Media
JournalFrontiers in Microbiology
CitationMina D, Pereira JA, Lino-Neto T and Baptista P (2020) Screening the Olive Tree Phyllosphere: Search and Find Potential Antagonists Against Pseudomonas savastanoi pv. savastanoi. Front. Microbiol. 11:2051. doi: 10.3389/fmicb.2020.02051
Abstract(s)Olive knot (OK) is a widespread bacterial disease, caused byPseudomonas savastanoipv.savastanoi(Pss), which currently has not effective control methods. The use of naturally occurring microbial antagonists, such as bacteria, as biocontrol agents could be a strategy to manage this disease. The objective of this work was to select bacteria from olive tree phyllosphere able to antagonizePssusingin vitroandin plantaexperiments. The elucidation of their modes of action and the potential relationship between antagonism and bacteria origin has been investigated, as well. To this end, 60 bacterial isolates obtained from the surface and inner tissues of different organs (leaves, twigs, and knots), from two olive cultivars of varying susceptibilities to OK, were screened for theirin vitroantagonistic effect againstPss. A total of 27 bacterial strains were able to significantly inhibitPssgrowth, being this effect linked to bacteria origin. Strains from OK-susceptible cultivar and colonizing the surface of plant tissues showed the strongest antagonistic potential. The antagonistic activity was potentially due to the production of volatile compounds, siderophores and lytic enzymes.Bacillus amyloliquefaciensP41 was the most effective antagonistic strain and their capacity to control OK disease was subsequently assayed usingin plantaexperiments. This strain significantly reduces OK disease severity (43.7%), knots weight (55.4%) and population size ofPss(26.8%), while increasing the shoot dry weight (55.0%) and root water content (39.6%) ofPss-infected olive plantlets. Bacterial isolates characterized in this study, in particularB. amyloliquefaciensP41, may be considered as promising biocontrol candidates for controlling OK disease.
TypeArticle
DescriptionThe Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2020.02051/full#supplementary-material
URIhttps://hdl.handle.net/1822/73134
DOI10.3389/fmicb.2020.02051
ISSN1664-302X
Publisher versionhttps://www.frontiersin.org/articles/10.3389/fmicb.2020.02051
Peer-Reviewedyes
AccessOpen access
Appears in Collections:CBFP - Artigos/Papers

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