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TitleCancer cells’ metabolism dynamics in renal cell carcinoma patients’ outcome: influence of GLUT-1-Related hsa-miR-144 and hsa-miR-186
Author(s)Morais, Mariana
Dias, Francisca
Nogueira, Inês
Leão, Anabela
Gonçalves, Nuno
Araújo, Luís
Granja, Sara Costa
Baltazar, Fátima
Teixeira, Ana L.
Medeiros, Rui
KeywordsRenal cell carcinoma
Warburg effect
Glucose transporter 1
Issue date6-Apr-2021
PublisherMultidisciplinary Digital Publishing Institute (MDPI)
CitationMorais, M.; Dias, F.; Nogueira, I.; Leão, A.; Gonçalves, N.; Araújo, L.; Granja, S.; Baltazar, F.; Teixeira, A.L.; Medeiros, R. Cancer Cells’ Metabolism Dynamics in Renal Cell Carcinoma Patients’ Outcome: Influence of GLUT-1-Related hsa-miR-144 and hsa-miR-186. Cancers 2021, 13, 1733.
Abstract(s)The cancer cells’ metabolism is altered due to deregulation of key proteins, including glucose transporter 1 (GLUT-1), whose mRNA levels are influenced by microRNAs (miRNAs). Renal cell carcinoma (RCC) is the most common and lethal neoplasia in the adult kidney, mostly due to the lack of accurate diagnosis and follow-up biomarkers. Being a metabolic associated cancer, this study aimed to understand the hsa-miR-144-5p and hsa-miR-186-3p’s potential as biomarkers of clear cell RCC (ccRCC), establishing their role in its glycolysis status. Using three ccRCC lines, the intra- and extracellular levels of both miRNAs, GLUT-1’s mRNA expression and protein levels were assessed. Glucose consumption and lactate production were evaluated as glycolysis markers. A decrease of intracellular levels of these miRNAs and increase of their excretion was observed, associated with an increase of GLUT-1’s levels and glycolysis’ markers. Through a liquid biopsy approach, we found that RCC patients present higher plasmatic levels of hsa-miR-186-3p than healthy individuals. The Hsa-miR144-5p’s higher levels were associated with early clinical stages. When patients were stratified according to miRNAs plasmatic levels, low plasmatic levels of hsa-miR-144-5p and high plasmatic levels of hsa-miR-186-3p (high-risk group) showed the worst overall survival. Thus, circulating levels of these miRNAs may be potential biomarkers of ccRCC prognosis.
Publisher version
AccessOpen access
Appears in Collections:BUM - MDPI

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