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TitleL-lactic acid production from multi-supply autohydrolyzed economically unexploited lignocellulosic biomass
Author(s)Pontes, Rita
Romani, Aloia Perez
Michelin, Michele
Domingues, Lucília
Teixeira, J. A.
Nunes, João
KeywordsLignocellulosic biomass mixture
Simultaneous saccharification and fermentation
Lactic acid
Lactobacillus rhamnosus
Issue date15-Oct-2021
JournalIndustrial Crops and Products
CitationPontes, Rita; Romaní, Aloia; Michelin, Michele; Domingues, Lucília; Teixeira, José A.; Nunes, João, L-lactic acid production from multi-supply autohydrolyzed economically unexploited lignocellulosic biomass. Industrial Crops and Products, 170(113775), 2021
Abstract(s)The present paradigm of a new concept of biomass conversion system has been focused on the development of a multi-supply biorefinery concept towards a circular and sustainable bioeconomy. This work was focused on evaluating a mixture of six lignocellulosic biomass types, from forest ecosystems and biological resources from marginal land, in order to produce lactic acid (L-LA) by Lactobacillus rhamnosus within a multi-supply biorefinery scheme. The mixture of lignocellulosic biomass was submitted to autohydrolysis pretreatment under non-isothermal regime at 226 °C (corresponding to a severity of 4.15) yielding a glucan recovery of 93 % in the solid phase. Two different strategies were assayed for L-LA production, namely separated hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF). The glucose to LA yield obtained for both assays was 1 g/g, although the volumetric productivity of SSF (2.5 g/Lh) was higher than SHF (0.8 g/Lh). Therefore, the SSF process was optimized through a factorial design to evaluate the effect of the independent variables, solids load (SL) and enzyme-substrate ratio (ESR), on LA production. The maximum concentration of LA was obtained using the highest solids load (16 %) and with the highest ESR (54 FPU/g). Finally, L-LA was produced using in a bioreactor under the optimized conditions from experimental design, obtaining 61.74 g/L of LA at 44 h, with an optical purity of 98 % and a LA yield of 0.97 g/g. This study showed the potential of multi-supply unexploited lignocellulosic biomass as a raw material to LA fermentation.
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Appears in Collections:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series

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