Utilize este identificador para referenciar este registo: https://hdl.handle.net/1822/73522

TítuloFractionating stem cells secretome for Parkinson's disease modeling: Is it the whole better than the sum of its parts?
Autor(es)Vilaça-Faria, Helena
Marote, Ana
Lages, Inês
Ribeiro, Clarisse
Mendes-Pinheiro, Bárbara
Domingues, Ana Verónica
Campos, Jonas
Lanceros-Méndez, S.
Salgado, A. J.
Teixeira, Fábio G.
Palavras-chaveParkinson's disease
Mesenchymal stem cells
Secretome
Vesicles
DataOut-2021
EditoraElsevier
RevistaBiochimie
CitaçãoVilaça-Faria, H., Marote, A., Lages, I., Ribeiro, C., Mendes-Pinheiro, B., Domingues, A. V., . . . Teixeira, F. G. (2021). Fractionating stem cells secretome for Parkinson's disease modeling: Is it the whole better than the sum of its parts? Biochimie, 189, 87-98. doi: https://doi.org/10.1016/j.biochi.2021.06.008
Resumo(s)Human mesenchymal stem cells (hMSCs) secretome has been have been at the forefront of a new wave of possible therapeutic strategies for central nervous system neurodegenerative disorders, as Parkinson's disease (PD). While within its protein fraction, several promising proteins were already identified with therapeutic properties on PD, the potential of hMSCs-secretome vesicular fraction remains to be elucidated. Such highlighting is important, since hMSCs secretome-derived vesicles can act as biological nanoparticles with beneficial effects in different pathological contexts. Therefore, in this work, we have isolated hMSCs secretome vesicular fraction, and assessed their impact on neuronal survival, and differentiation on human neural progenitors' cells (hNPCs), and in a 6-hydroxydopamine (6-OHDA) rat model of PD when compared to hMSCs secretome (as a whole) and its protein derived fraction. From the results, we have found hMSCs vesicular fraction as polydispersity source of vesicles, which when applied in vitro was able to induce hNPCs differentiation at the same levels as the whole secretome, while the protein separated fraction was not able to induce such effect. In the context of PD, although distinct effects were observed, hMSCs secretome and its derived fractions displayed a positive impact on animals' motor and histological performance, thereby indicating that hMSCs secretome and its different fractions may impact different mechanisms and pathways. Overall, we concluded that the use of the secretome collected from hMSCs and its different fractions might be active modulators of different neuroregeneration mechanisms, which could open new therapeutical opportunities for their future use as a treatment for PD.
TipoArtigo
URIhttps://hdl.handle.net/1822/73522
DOI10.1016/j.biochi.2021.06.008
ISSN0300-9084
Versão da editorahttps://www.sciencedirect.com/science/article/pii/S0300908421001541
Arbitragem científicayes
AcessoAcesso restrito UMinho
Aparece nas coleções:CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series
ICVS - Artigos em revistas internacionais / Papers in international journals
FUNCTIONAL AND SMART MATERIALS AND SURFACES FOR ADVANCED APPLICATIONS (2018 - ...)

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