Polyunsaturated fatty acids production by solid-state fermentation on polyurethane foam by Mortierella alpina

Abstract

Polyunsaturated fatty acids (PUFAs) are essential in healthy diets and their production is extremely important. Natural sources of PUFAs includes animal and aquatic products such as marine fish oil, however there are several limitations such as the decrease of fish stocks throughout the world. Thus, microbial oils are a preferable source of PUFAs. Herein, it was studied the production of PUFAs by Mortierella alpina under solidstate fermentation (SSF) using polyurethane foam as inert substrate and synthetic medium or lignocellulosic hydrolysate as source of C, N and other nutrients. Several parameters of fermentation conditions were evaluated as carbon source, inductors addition, ratio C/N and temperature.

The highest amount of total PUFAs per mass of solid (535.41 ±24.12 mg/g), linoleic acid (129.66 ±5.84 mg/g) and linoleic acid (401.93 ±18.10 mg/g) were produced when the culture medium contained 20 g/L glucose, 10% (w/v) linseed oil, the C/N ratio was adjusted to 25 and the incubation temperature was 25 °C for 3days decreasing to 16 °C on the remaining 4days of fermentation. In addition, a hemicellulosic hydrolysate can be used as lowcost substrate to produce PUFAs, although the production was lower than the achieved with synthetic medium. SSF showed an interesting technology for microbial PUFAs production.