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https://hdl.handle.net/1822/73797
Título: | Galactose to tagatose isomerization by the l-arabinose isomerase from Bacillus subtilis: a biorefinery approach for Gelidium sesquipedale valorisation |
Autor(es): | Baptista, Sara Isabel Leite Romaní, Aloia Oliveira, Carla Cristina Marques de Ferreira, Sara Rocha, Cristina M.R. Domingues, Lucília |
Palavras-chave: | Tagatose l-arabinose isomerase Red macroalgae Biorefinery approach Gelidium sesquipedale |
Data: | Nov-2021 |
Editora: | Elsevier |
Revista: | LWT - Food Science and Technology |
Citação: | Sara Baptista; Romaní, Aloia; Oliveira, Carla; Ferreira, Sara; Rocha, Cristina M. R.; Domingues, Lucília, Galactose to tagatose isomerization by the l-arabinose isomerase from Bacillus subtilis: A biorefinery approach for Gelidium sesquipedale valorisation. LWT-Food Science and Technology, 151(112199), 2021 |
Resumo(s): | Tagatose is a rare sugar with increasing commercial interest as sweetener. Biotechnological production of d-tagatose by enzymatic isomerization of d-galactose provides an alternative to chemical processes. In the last years, l-arabinose isomerases (L-AIs) from different origins have been studied to increase the effectiveness of tagatose production. In this work, the L-AI from Bacillus subtilis, previously reported to have unique substrate specificity for l-arabinose, was expressed in Escherichia coli and studied for isomerization of d-galactose to d-tagatose. The recombinant enzyme demonstrated, for the first time, tagatose bioconversion capacity, reaching ~59% conversion. Furthermore, a sustainable tagatose production strategy was developed by using Gelidium sesquipedale red seaweed and its undervalued processing residues as source of galactose. L-AI successfully converted the galactose-rich hydrolysate, obtained from direct acid hydrolysis of seaweed, to tagatose (50.9% conversion). Additionally, the process combining autohydrolysis of G. sesquipedale and acid hydrolysis of the remaining residue allowed a full integral valorisation of polysaccharides: 13.33 g of agar, an important hydrocolloid, coupled with the production of 5.97 g of tagatose. These results confirmed that seaweed biomass and waste-derived are promising substrates for tagatose production by L-AI, contributing to the advancement of circular economy and to the actual needs of food industry. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/73797 |
DOI: | 10.1016/j.lwt.2021.112199 |
ISSN: | 0023-6438 |
Versão da editora: | https://www.sciencedirect.com/science/article/pii/S0023643821013529 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
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document_54550_1.pdf Acesso restrito! | 2,61 MB | Adobe PDF | Ver/Abrir |