Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/73909
Título: | Chemical characterization of Sambucus nigra L. flowers aqueous extract and its biological implications |
Autor(es): | Ferreira-Santos, P. Costa, H. Salvador, Ângelo C. Silvestre, Armando J. D. Santos, Sónia A. O. Rocha, Sílvia M. Sousa, Ana Margarida Pereira, Maria Olívia Pereira-Wilson, Cristina Rocha, Cristina M.R. Teixeira, J. A. Botelho, C. M. |
Palavras-chave: | Elderflower GC-MS HPLC-MS Chemical profile Phenolic compounds Cytotoxicity Antibacterial activity Antioxidant activity |
Data: | 17-Ago-2021 |
Editora: | Multidisciplinary Digital Publishing Institute (MDPI) |
Revista: | Biomolecules |
Citação: | Ferreira-Santos, P.; Costa, H.; Salvador, Ângelo C.; Silvestre, Armando J. D.; Santos, Sónia A. O.; Rocha, Sílvia M.; Sousa, Ana Margarida; Pereira, Maria Olívia; Pereira-Wilson, Cristina; Rocha, Cristina M. R.; Teixeira, José A.; Botelho, Cláudia M., Chemical characterization of Sambucus nigra L. flowers aqueous extract and its biological implications. Biomolecules, 11(8), 1222, 2021 |
Resumo(s): | The main goal of this study was to chemically characterize an aqueous S. nigra flower extract and validate it as a bioactive agent. The elderflower aqueous extraction was performed at different temperatures (50, 70 and 90 °C). The extract obtained at 90 °C exhibited the highest phenolic content and antiradical activity. Therefore, this extract was analyzed by GC-MS and HPLC-MS, which allowed the identification of 46 compounds, being quercetin and chlorogenic acid derivatives representative of 86% of the total of phenolic compounds identified in hydrophilic fraction of the aqueous extract. Naringenin (27.2%) was the major compound present in the lipophilic fraction. The antiproliferative effects of the S. nigra extract were evaluated using the colon cancer cell lines RKO, HCT-116, Caco-2 and the extracts antigenotoxic potential was evaluated by the Comet assay in RKO cells. The RKO cells were the most susceptible to S. nigra flower extract (IC50=1250 µg mL1). Moreover, the extract showed antimicrobial activity against Gram-positive bacteria, particularly Staphylococcus aureus and S. epidermidis. These results show that S. nigra-based extracts can be an important dietary source of bioactive phenolic compounds that contribute to health-span improving life quality, demonstrating their potential as nutraceutical, functional foods and/or cosmetic components for therapeutic purposes. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/73909 |
DOI: | 10.3390/biom11081222 |
e-ISSN: | 2218-273X |
Versão da editora: | https://www.mdpi.com/2218-273X/11/8/1222 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series DBio - Artigos/Papers |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_54582_1.pdf | 2,2 MB | Adobe PDF | Ver/Abrir |
Este trabalho está licenciado sob uma Licença Creative Commons