Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/74015
Título: | Colorimetric cellulose-based test-strip for rapid detection of amyloid -42 |
Autor(es): | Moreira, Felismina Correia, Bárbara Sousa, Mariana Sales, M. G. F. |
Palavras-chave: | Test strips Naked-eye detection Molecularlyimprinted polymers Cellulose substrates Colorimetricmeasurements |
Data: | 8-Set-2021 |
Editora: | Springer Nature |
Revista: | Microchimica Acta |
Citação: | Moreira, Felismina; Correia, Bárbara; Sousa, Mariana; Sales, M. G. F., Colorimetric cellulose-based test-strip for rapid detection of amyloid -42. Microchimica Acta, 188(334), 2021 |
Resumo(s): | An innovative sensing assay is described for point-of-care (PoC) quantification of a biomarker of Alzheimers disease, amyloid -42 (A-42). This device is based on a cellulose paper-dye test strip platform in which the corresponding detection layer is integrated by applying a molecularly imprinted polymer (MIP) to the cellulose paper surface. Briefly, the cellulose paper is chemically modified with a silane to subsequently apply the MIP detection layer. The imprinting process is confirmed by the parallel preparation of a control material, namely a non-imprinted polymer (NIP). The chemical changes of the surface were evaluated by Fourier transform infrared spectroscopy (FTIR), contact angle, and thermogravimetric analysis (TG). Proteins and peptides can be quantified by conventional staining methods. For this purpose, Coomassie blue (CB) was used as a staining dye for the detection and quantification of A-42. Quantitative determination is made possible by taking a photograph and applying an appropriate mathematical treatment to the color coordinates provided by the ImageJ program. The MIP shows a linear range between 1.0 ng/mL and 10 g/mL and a detection limit of 0.71 ng/mL. Overall, this cellulose-based assay is suitable for the detection of peptides or proteins in a sample by visual comparison of color change. The test strip provides a simple, instrument-free, and cost-effective method with high chemical stability, capable of detecting very small amounts of peptides or proteins in a sample, and can be used for the detection of any (bio)molecule of interest. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/74015 |
DOI: | 10.1007/s00604-021-04996-7 |
ISSN: | 0026-3672 |
Versão da editora: | https://link.springer.com/article/10.1007%2Fs00604-021-04996-7 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_54631_1.pdf Acesso restrito! | 1,7 MB | Adobe PDF | Ver/Abrir |