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https://hdl.handle.net/1822/74508
Título: | FISH Variants |
Autor(es): | Guimarães, Nuno M. Azevedo, Nuno F. Almeida, Carina |
Palavras-chave: | FISH CARD-FISH Gene-FISH Phage-FISH MAR-FISH FISH-NanoSIMS CLASI-FISH DOPE-FISH Leaf-FISH Flow-FISH Microfluidic-FISH NAM-FISH QD-FISH NAM-FISH QD-FISH |
Data: | 13-Fev-2021 |
Editora: | Springer US |
Revista: | Methods in Molecular Biology |
Citação: | Guimarães, Nuno M.; Azevedo, Nuno F.; Almeida, Carina, FISH Variants. In Nuno F. Azevedo, Carina Almeida, Fluorescence In-Situ Hybridization (FISH) for Microbial Cells: Methods and Concepts, Vol. Methods in Molecular Biology 2246, Springer US, 2021. ISBN: 978-1-0716-1115-9, 17-33. DOI: 10.1007/978-1-0716-1115-9_2 |
Resumo(s): | FISH has gained an irreplaceable place in microbiology because of its ability to detect and locate a microorganism, or a group of organisms, within complex samples. However, FISH role has evolved drastically in the last few decades and its value has been boosted by several advances in signal intensity, imaging acquisitions, automation, method robustness, and, thus, versatility. This has resulted in a range of FISH variants that gave researchers the ability to access a variety of other valuable information such as complex population composition, metabolic activity, gene detection/quantification, or subcellular location of genetic elements. In this chapter, we will review the more relevant FISH variants, their intended use, and how they address particular challenges of classical FISH. |
Tipo: | Capítulo de livro |
URI: | https://hdl.handle.net/1822/74508 |
ISBN: | 978-1-0716-1114-2 |
e-ISBN: | 978-1-0716-1115-9 |
DOI: | 10.1007/978-1-0716-1115-9_2 |
ISSN: | 1064-3745 |
Versão da editora: | https://link.springer.com/book/10.1007/978-1-0716-1115-9 |
Arbitragem científica: | yes |
Acesso: | Acesso restrito UMinho |
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document_54192_1.pdf Acesso restrito! | 257,25 kB | Adobe PDF | Ver/Abrir |