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https://hdl.handle.net/1822/7472
Título: | Isolation and purification of an enzyme hydrolyzing ochratoxin A from aspergillus niger |
Autor(es): | Abrunhosa, Luís Venâncio, Armando |
Palavras-chave: | Aspergillus niger Carboxypeptidase Degradation Ochratoxin A Ochratoxin α |
Data: | Dez-2007 |
Editora: | Springer |
Revista: | Biotechnology Letters |
Citação: | "Biotechnology Letters." ISSN 0141-5492. 29:12 (Dez. 2007) p. 1909-1914. |
Resumo(s): | Ochratoxin A is a mycotoxin produced by several Aspergillus and some Penicillium species which may be present in food and feed products. It can be enzymatically hydrolyzed into ochratoxin α and l-β-phenylalanine, thereby decreasing its toxicity. The ochratoxin A degradation capacity of Aspergillus niger is well known and here we report the isolation and purification of a novel enzyme from A. niger that hydrolyzes this mycotoxin. A wheat germ medium supplemented with ochratoxin A was used to produce the enzyme, which was purified from culture filtrate by acetone precipitation and anion exchange chromatography. An overall purification of 2.5-fold with a recovery of 68% and a final specific activity of 36 U/mg was obtained. The enzyme is a metalloenzyme as it was inhibited at 10 mM EDTA, whereas PMSF had no effect. The ochratoxin A hydrolytic enzyme presented a V max of 0.44 μM/min and a K m of 0.5 mM when the reaction was carried out at pH 7.5 and 37°C. |
Tipo: | Artigo |
URI: | https://hdl.handle.net/1822/7472 |
DOI: | 10.1007/s10529-007-9479-2 |
ISSN: | 0141-5492 1573-6776 |
Arbitragem científica: | yes |
Acesso: | Acesso aberto |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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Abrunhosa_Biotechnol_Letters.pdf | 270,35 kB | Adobe PDF | Ver/Abrir |