Utilize este identificador para referenciar este registo:
https://hdl.handle.net/1822/74818
Registo completo
Campo DC | Valor | Idioma |
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dc.contributor.author | Araújo, Daniela | por |
dc.contributor.author | Braz, Joana | por |
dc.contributor.author | Dencheva, Nadya Vasileva | por |
dc.contributor.author | Carvalho, Isabel | por |
dc.contributor.author | Henriques, Mariana | por |
dc.contributor.author | Denchev, Z. | por |
dc.contributor.author | Malfois, Marc | por |
dc.contributor.author | Silva, Sónia Carina | por |
dc.date.accessioned | 2021-11-30T11:51:44Z | - |
dc.date.issued | 2021-04-30 | - |
dc.identifier.citation | Araújo, Daniela; Braz, Joana; Dencheva, Nadya V.; Carvalho, Isabel; Henriques, Mariana; Denchev, Zlatan Z.; Malfois, Marc; Silva, Sónia Carina, Polyamide microsized particulate polyplex carriers for the 2-OMethylRNA EFG1 antisense oligonucleotide. ACS Applied Bio Materials, 4(5), 4607-4617, 2021 | por |
dc.identifier.issn | 2576-6422 | por |
dc.identifier.uri | https://hdl.handle.net/1822/74818 | - |
dc.description.abstract | Anti-EFG1 2-OMethylRNA is an antisense oligonucleotide (ASO) that has the ability to recognize and block the EFG1 gene and to control Candida albicans filamentation. However, it is important to protect the anti-EFG1 2-OMethylRNA ASO from the environmental human body conditions and to ensure that they will be delivered to their site of action, and polyplex microparticles (MPs) represent a class of vehicles to ASO cargo with these functionalities. Thus, the goal of this work was to develop polyplexes based on porous poly(-butyrolactam) (PA4) or poly(-caprolactam) (PA6) MPs for the anti-EFG1 2-OMethylRNA ASO cargo and delivery. Two types of polyplexes were prepared with payloads of anti-EFG1 2-OMethylRNA molecules, either entrapped or immobilized on prefabricated polyamide MPs. Our data confirm that PA4 and PA6 polyplex MPs can be feasible carriers for anti-EFG1 2-OMethylRNA ASO molecules, using either the entrapment or immobilization strategies, whereby the released ASO maintains its activity against C. albicans cells. | por |
dc.description.sponsorship | This study was supported by the Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of the UIDB/04469/2020 unit and BioTecNorte operation (NORTE-01-0145-FEDER-000004) funded by the European Regional Development Fund under the scope of Norte2020Programa Operacional Regional do Norte and Daniela Eira Araujo [SFRH/BD/121417/2016] Ph.D. grant. The authors also acknowledge the project funding by the “02/SAICT/2017 Projetos de Investigação Científica e Desenvolvimento Tecnológico (IC&DT) POCI-01-0145-FEDER-028893”. N.V.D. and Z.Z.D. are also grateful to the Executive Commission of ALBA synchrotron for the support under the project no. 2018022726. | por |
dc.language.iso | eng | por |
dc.publisher | American Chemical Society | por |
dc.relation | SFRH/BD/121417/2016 | por |
dc.rights | restrictedAccess | por |
dc.subject | Degradation | por |
dc.subject | Amides | por |
dc.subject | Organic polymers | por |
dc.subject | X-ray scattering | por |
dc.subject | Molecules | por |
dc.subject | Candida albicans filamentation | por |
dc.subject | antisense oligonucleotides | por |
dc.subject | polyplex RNA carriers | por |
dc.subject | polyamide 4 | por |
dc.subject | polyamide 6 | por |
dc.subject | polymer microparticles | por |
dc.title | Polyamide microsized particulate polyplex carriers for the 2-OMethylRNA EFG1 antisense oligonucleotide | por |
dc.type | article | - |
dc.peerreviewed | yes | por |
dc.relation.publisherversion | https://pubs.acs.org/doi/10.1021/acsabm.1c00334 | por |
dc.comments | CEB54400 | por |
oaire.citationStartPage | 4607 | por |
oaire.citationEndPage | 4617 | por |
oaire.citationIssue | 5 | por |
oaire.citationConferencePlace | United States | - |
oaire.citationVolume | 4 | por |
dc.date.updated | 2021-10-09T11:44:45Z | - |
dc.identifier.doi | 10.1021/acsabm.1c00334 | por |
dc.date.embargo | 10000-01-01 | - |
dc.identifier.pmid | 35006798 | por |
dc.description.publicationversion | info:eu-repo/semantics/publishedVersion | - |
dc.subject.wos | Science & Technology | por |
sdum.journal | ACS Applied Bio Materials | por |
Aparece nas coleções: | CEB - Publicações em Revistas/Séries Internacionais / Publications in International Journals/Series |
Ficheiros deste registo:
Ficheiro | Descrição | Tamanho | Formato | |
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document_54400_1.pdf Acesso restrito! | 7,97 MB | Adobe PDF | Ver/Abrir |